Imperial/Wet Lab/Lab Notebook/2007-09-28

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(28 September 2007)
 
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==Optimum DNA concentration==
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*Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct.
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*The constructs were tested for 0, 1, 2, 4 and 6ug of DNA.
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*The fluorescence over a period of 6 hours was recorded every hour for all the concentrations.
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*It was found that at 25&deg;C pTet-lixR-pLux-GFP behaves as expected, i.e. the optimum output is for a mass between 2 and 4ug of DNA.
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*For pTet-GFP construct, at 37&deg;C, the fluorecence was highest at the end of the day for 6ug of DNA.
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Latest revision as of 14:13, 10 October 2007

28 September 2007

Optimum DNA concentration

  • Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct.
  • The constructs were tested for 0, 1, 2, 4 and 6ug of DNA.
  • The fluorescence over a period of 6 hours was recorded every hour for all the concentrations.
  • It was found that at 25°C pTet-lixR-pLux-GFP behaves as expected, i.e. the optimum output is for a mass between 2 and 4ug of DNA.
  • For pTet-GFP construct, at 37°C, the fluorecence was highest at the end of the day for 6ug of DNA.