Imperial/Wet Lab/Lab Notebook/2007-10-16

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==pT7-GFPmut3b chracterisation==
==pT7-GFPmut3b chracterisation==
*The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
*The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
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*The reactions were sampled every hour
*The reactions were sampled every hour
*The [[Imperial/Wet_Lab/Results/Res1.9|Results]] showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures
*The [[Imperial/Wet_Lab/Results/Res1.9|Results]] showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures
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Latest revision as of 18:55, 26 October 2007


pT7-GFPmut3b chracterisation

  • The aim of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
  • For materials and methods see the Protocol
  • The construct was tested for three temperatures: 4°C, 25°C and 37°C
  • The reactions were sampled every hour
  • The Results showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures