Imperial/Wet Lab/Lab Notebook/2007-10-19

From 2007.igem.org

(Difference between revisions)
Line 1: Line 1:
 +
<html></div></html>
 +
{{Template:IC07labnotebook}}
 +
==AHL titration curve for infector detector construct==
==AHL titration curve for infector detector construct==
*The '''aim'' of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations
*The '''aim'' of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations

Revision as of 18:57, 26 October 2007

AHL titration curve for infector detector construct

  • The 'aim of this experiment was to test the pTet-luxR-pLux-GFPmut3b construct with a range of AHL concentrations. A titration curve could then be created for the construct in terms of AHL concentrations
  • The materials and methods can be found in the Protocols
  • The concentrations of AHL for which the construct was tested are: 0nM, 5nM, 10nM, 15nM, 20nM, 50nM, 100nM, 1000nM
  • There were two negative controls for the experiment:
    • Empty vector with [AHL]=1000nM
    • pTet-luxR-pLux-GFPmut3b construct with [AHL] = 0nM
  • The Results showed that the total fluorescent output produced by the construct increases with increasing [AHL]