Imperial/Wet Lab/Protocols

From 2007.igem.org

< Imperial | Wet Lab(Difference between revisions)
(Wet Lab: Protocols)
 
(35 intermediate revisions not shown)
Line 1: Line 1:
 +
<br clear="all">
 +
{{Template:IC07navmenu}}
{{Template:IC07navmenu}}
__NOTOC__
__NOTOC__
 +
<br clear="all">
= Wet Lab: Protocols =
= Wet Lab: Protocols =
Below is a databank of all the protocols that have been used in our projects.  
Below is a databank of all the protocols that have been used in our projects.  
Line 12: Line 15:
|
|
<font size=2><font color=green>'''Protein Purification'''</font> <br>
<font size=2><font color=green>'''Protein Purification'''</font> <br>
-
* ''Purification of LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]
+
* ''Purification of LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.5|Results]]  
-
* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.6 Results]
+
* ''Purification of GFPmut3b'':  [[Imperial/Wet_Lab/Protocols/Prot1.6|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.6|Results]]
<br>
<br>
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
<font size=2><font color=green>'''Fluorometer Testing'''</font> <br>
-
* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.1 Results]<br>
+
* ''Optimisation of Fluorometer'': [[Imperial/Wet_Lab/Protocols/Prot1.1|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.1|Results]]<br>
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
<font size=2><font color=green>'''Calibration Curve'''</font> <br>
-
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.3 Results]
+
* ''Calibration Curve for GFPmut3b'': [[Imperial/Wet_Lab/Protocols/Prot1.3|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.3|Results]]
-
* ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/Res1.4 Results]
+
* ''Degradation Curve for GFPmut3b'':[[Imperial/Wet_Lab/Protocols/Prot1.4|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.4|Results]]
<br>
<br>
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
-
* ''Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
+
* ''Reaction Mixture of Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
-
* ''Preparation S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
+
* ''Reaction Mixture of Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]]
-
* ''Preparation S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
+
* ''Recipe for S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
-
* ''Vesicles Formation'':[[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
+
* ''Recipe for S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
 +
* ''Testing of Homemade S30 Cell Extract with ID construct '':[[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1|Results]]<br>
 +
* ''Vesicles Formation'': [[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
<br>
<br>
|
|
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
<font size=2><font color=green>'''pT7 Characterisation'''</font> <br>
-
*''pT7 in vitro:'' [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/Protocols/Prot1.9 Protocols] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.9 Results]
+
*''pT7 in vitro:'' [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] and [[Imperial/Wet_Lab/Results/Res1.9|Results]]
<br>
<br>
<font size=2>'''Cell by Date'''</font> <br>
<font size=2>'''Cell by Date'''</font> <br>
<font color=green>'''Implementation'''</font><br>
<font color=green>'''Implementation'''</font><br>
-
* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.1 Results]
+
* ''In Vivo Testing:'' [[Imperial/Wet_Lab/Protocols/CBD1.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.1|Results]]
-
* ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD1.2 Results]
+
* ''Initial In vitro Testing'': [[Imperial/Wet_Lab/Protocols/CBD1.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD1.2|Results]]
<font color=green>'''Testing/Validation<br>'''</font>
<font color=green>'''Testing/Validation<br>'''</font>
-
*''Testing DNA Concentration in vitro'': [[IGEM:IMPERIAL/2007/Wet_Lab/Protocols/Prot1.8|Protocols]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD4 Results]
+
*''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
-
* ''Operating Temperature Range'': [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocol 1]] and [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocol 2]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.2 Results 1] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.1 Results 2]
+
* ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]]
-
*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/CBD2.3 Results]
+
*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]]  
 +
*''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]]
<br>
<br>
-
<font size=2>'''Infecter Detector'''</font> <br>
+
<font size=2>'''Infector Detector'''</font> <br>
<font color=green>'''Implementation<br>'''</font>
<font color=green>'''Implementation<br>'''</font>
-
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]
+
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]]
-
* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.2 Results]
+
* ''Initial In Vitro Testing'': [[Imperial/Wet_Lab/Protocols/ID1.2|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.2|Results]]
<font color=green>'''Testing/Validation<br>'''</font>
<font color=green>'''Testing/Validation<br>'''</font>
-
*''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res2.1 Results]
+
*''Testing of DNA concentration'' : [[Imperial/Wet_Lab/Protocols/ID2.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID2.1|Results]]
-
* ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]
+
* ''Testing of Purified LuxR'': [[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.7|Results]]
-
* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/ID3.1 Results]
+
* ''Characterization in vitro - AHL titration '': [[Imperial/Wet_Lab/Protocols/ID3.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID3.1|Results]]
|}
|}
<br clear="all">
<br clear="all">
 +
 +
 +
<center>| [[Imperial/Wet_Lab| Wet Lab >>]]</center>

Latest revision as of 02:15, 27 October 2007




Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols

  • Reaction Mixture of Commercially available S30 Cell Extract :Protocol
  • Reaction Mixture of Homemade S30 Cell Extract :Protocol
  • Recipe for S30 Cell Extract :Protocol
  • Recipe for S12 Cell Extract :Protocol
  • Testing of Homemade S30 Cell Extract with ID construct :Protocol and Results
  • Vesicles Formation: Protocol


pT7 Characterisation


Cell by Date
Implementation

Testing/Validation


Infector Detector
Implementation

Testing/Validation



| Wet Lab >>