Imperial/Wet Lab/Protocols

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= Wet Lab: Protocols =
= Wet Lab: Protocols =
Below is a databank of all the protocols that have been used in our projects.  
Below is a databank of all the protocols that have been used in our projects.  
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<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
<font size=2><font color=green>'''Chassis Protocols'''</font> <br>
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* ''Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
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* ''Reaction Mixture of Commercially available S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.3|Protocol]]
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* ''Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]]
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* ''Reaction Mixture of Homemade S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.4|Protocol]]
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* ''Preparation S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
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* ''Recipe for S30 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.1|Protocol]]
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* ''Preparation S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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* ''Recipe for S12 Cell Extract '':[[Imperial/Wet_Lab/Protocols/CE1.2|Protocol]]
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* ''Vesicles Formation'':[[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
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* ''Testing of Homemade S30 Cell Extract with ID construct '':[[Imperial/Wet_Lab/Protocols/CE1.5|Protocol]] and [[Imperial/Wet_Lab/Results/Chas1.1|Results]]<br>
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* ''Vesicles Formation'': [[Imperial/Wet_Lab/Protocols/VF1.1|Protocol]]
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*''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
*''Testing DNA Concentration in vitro'': [[Imperial/Wet_Lab/Protocols/CBD1.3|Protocols]] and [[Imperial/Wet_Lab/Results/Res1.8|Results]]
* ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]]
* ''Isothermal experiments'': [[Imperial/Wet_Lab/Protocols/CBD2.2|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.2|Results]]
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*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocol]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]]  
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*''Temperature Steps:'' [[Imperial/Wet_Lab/Protocols/CBD2.3|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.3|Results]]  
*''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]]
*''Packaging experments:'' [[Imperial/Wet_Lab/Protocols/CBD2.1|Protocols]] and [[Imperial/Wet_Lab/Results/CBD2.1|Results]]
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<font size=2>'''Infecter Detector'''</font> <br>
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<font size=2>'''Infector Detector'''</font> <br>
<font color=green>'''Implementation<br>'''</font>
<font color=green>'''Implementation<br>'''</font>
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]]
*''In vivo Testing'': [[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [[Imperial/Wet_Lab/Results/ID1.1|Results]]
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<center>| [[Imperial/Wet_Lab| Wet Lab >>]]</center>

Latest revision as of 02:15, 27 October 2007




Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification


Fluorometer Testing

Calibration Curve


Chassis Protocols

  • Reaction Mixture of Commercially available S30 Cell Extract :Protocol
  • Reaction Mixture of Homemade S30 Cell Extract :Protocol
  • Recipe for S30 Cell Extract :Protocol
  • Recipe for S12 Cell Extract :Protocol
  • Testing of Homemade S30 Cell Extract with ID construct :Protocol and Results
  • Vesicles Formation: Protocol


pT7 Characterisation


Cell by Date
Implementation

Testing/Validation


Infector Detector
Implementation

Testing/Validation



| Wet Lab >>