Ljubljana/styletest

From 2007.igem.org

< Ljubljana(Difference between revisions)
 
(24 intermediate revisions not shown)
Line 15: Line 15:
     </div>
     </div>
     <div id="about">
     <div id="about">
-
       <h3><span>About us </span></h3>
+
       <h3><span></span></h3>
-
       <p class="p1"><span>Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.</span></p>
+
       <p class="p1"><span><b>Synthetic biology provides the possibility to extend our defense against disease by employing our intelligence. In the spirit of synthetic biology we can combine different functional parts to assemble new cellular functions which are not yet existing in nature.</b></span></p>
-
      <p class="p2"><span>Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua consetetur sadipscing elitr.</span></p>
+
 
-
      <p class="p3"><span>Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. </span></p>
+
     </div>
     </div>
   </div>
   </div>
   <div id="supportingText">
   <div id="supportingText">
     <div id="products">
     <div id="products">
-
       <h3><span>Products</span></h3>
+
       <h3><span>Abstract</span></h3>
-
       <p class="p1"><span>Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est <a href="#">Lorem ipsum dolor sit amet.</a></span></p>
+
       <p class="p1"><span>HIV-1 virus is one of the most difficult targets for therapy because it hijacks our immune system and particularly because the virus mutates rapidly. This allows the viral armies to select the mutated variant strains which bypass the inhibitors that bind to specific residues on their targets. Current therapy consists of an inhibitor cocktail that decreases the probability of HIV to modify all target sequences at the same time.<br><br>
-
      <p class="p2"><span>Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua.</span></p>
+
 
-
    </div>
+
We propose a different strategy, where we target a specific <b>FUNCTION</b> of virus, rather than any particular sequence. This viral function triggers a cellular response which can either employ antiviral defense or lead to a destruction of infected cells to prevent spread of the infection.<br><br>
-
    <div id="services">
+
 
-
      <h3><span>Services</span></h3>
+
For implementation of this idea we have selected two functions: <b>viral attachment</b> to the cellular co-receptors CD4 and CCR5 and <b>viral proprotein processing</b> by its own protease.
-
      <p class="p1">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet <a href="#">clita kasd gubergren, no sea takimata</a> sanctus est Lorem ipsum dolor sit amet. </p>
+
 
-
      <p class="p2">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. </p>
+
Binding of virus to T-cells leads to the formation of CD4-CCR5 heterodimer. In our device, formation of this heterodimer triggers reconstitution of a split protein (split ubiquitin or TEVP) which activates the specific proteolytic activity. This releases the T7 RNA polymerase from the membrane anchor and leads to transcription of the effector gene, which prevents the spread of viral infection.<br>
-
      <p class="p3">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
The second implementation of this idea was to utilize the HIV-protease, which is required for viral maturation and cleaves a specific amino acid sequence. This target sequence was engineered between the membrane anchor and T7 polymerase. Activation of viral protease similarly as above releases the T7 polymerase and starts the defense program.<br><br>
-
    </div>
+
 
-
    <div id="support">
+
Important points of both approaches are to use the amplification of the signal, achieved by the use of T7 polymerase and the system's versatility, as we can select any number of different genes to be activated by the detection of viral function. The crucial aspect of our approach is that this system is <b>not sensitive to viral mutations</b> and is not activated only in case where the mutation leads to the loss of the viral function, which however also renders virus harmless at the same time.<br><br>
-
      <h3><span>Support</span></h3>
+
 
-
      <p class="p1">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
We have tested many different constructs, contributing more than 70 new BioBricks and successfully demonstrated on mammalian cell culture activation of response gene by infection with HIV-1 pseudovirus.<br><br>
-
    </div>
+
 
-
    <div id="development">
+
 
-
      <h3><span>Development</span></h3>
+
 
-
      <p class="p1">Lorem ipsum dolor sit amet, <a href="#">consetetur sadipscing elitr, sed diam nonumy</a> eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
<table cellspacing="0" cellpadding="0" border="1" style="border: medium none ; border-collapse: collapse;" class="MsoTableGrid">
-
      <p class="p2">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
<tbody>
-
      <p class="p3">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. </p>
+
  <tr style="">
-
      <p class="p4">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore magna aliquyam erat, sed diam voluptua. At vero eos et accusam et justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus est Lorem ipsum dolor sit amet. </p>
+
  <td width="614" valign="top" style="border: 1pt solid windowtext; padding: 0cm 5.4pt; width: 460.6pt;">
-
      <p class="p5">Lorem ipsum dolor sit amet, consetetur sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore.</p>
+
  <p class="MsoNormal"><o:p><b>PLEDGE:</b> All experimental work on this project was performed from May to October 2007 by the undergraduate students participating in the team under the tutorial of instructors. All the students participated at iGEM for the first time.</o:p></p>
-
    </div>
+
  </td>
-
     <div id="footer">&copy; Copyright Your Company Name<br />
+
  </tr>
-
       Designed by <a href="http://www.templateyes.com">TemplateYes</a></div>
+
</tbody>
 +
</table>
 +
 
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
     <div id="footer">______________________________________<br />
 +
       |<a href="https://2007.igem.org/Ljubljana">Home</a>|
 +
<a href="https://2007.igem.org/Ljubljana/AIDSplague">AIDS - Today's Plague</a>
 +
 
 +
 
 +
 
 +
</div>
   </div>
   </div>
   <div id="linkList">
   <div id="linkList">
     <div id="linkList2">
     <div id="linkList2">
       <div id="lmenu">
       <div id="lmenu">
-
         <h3 class="menu"><span><a class="two" href="#">HIV - Today's Plague</a></span></h3>
+
         <h3 class="menu"><span><a class="two" href="https://2007.igem.org/Ljubljana/AIDSplague">AIDS - Today's Plague</a></span></h3>
         <ul>
         <ul>
-
           <li><a class="one" href="#">AIDS Epidemic</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/HIVbacground">HIV-1 Infection Background</a>&nbsp; </li>
-
           <li><a class="one" href="#">Current Disease Treatment</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/Currenttreatment">Current Disease Treatment</a>&nbsp; </li>
         </ul>
         </ul>
       </div>
       </div>
       <div id="llinks">
       <div id="llinks">
-
         <h3 class="links"><span><a class="two" href="#">Our Idea</a></span></h3>
+
         <h3 class="links"><span><a class="two" href="https://2007.igem.org/Ljubljana/Strategy">Strategy</a></span></h3>
         <ul>
         <ul>
-
           <li><a class="one" href="#">Split Protein</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/implementation">Implementation</a>&nbsp; </li>
-
           <li><a class="one" href="#">HIV - Protease activity</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/model">Model</a>&nbsp; </li>
         </ul>
         </ul>
       </div>
       </div>
       <div id="lresources">
       <div id="lresources">
-
         <h3 class="resources"><span><a class="two" href="#">Project</a></span></h3>
+
         <h3 class="resources"><span><a class="two" href="https://2007.igem.org/Ljubljana/results">Results</a></span></h3>
         <ul>
         <ul>
-
           <li><a class="one" href="#">System</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/subsystems">Subsystems Testing</a>&nbsp; </li>
-
           <li><a class="one" href="#">Model</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/finalsystem">Performance of the Final Functional Systems</a>&nbsp; </li>
-
           <li><a class="one" href="#">Methods</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/biobricks">BioBricks</a>&nbsp; </li>
 +
          <li><a class="one" href="https://2007.igem.org/Ljubljana/methods">Methods</a>&nbsp; </li>
         </ul>
         </ul>
       </div>
       </div>
       <div id="lresults">
       <div id="lresults">
-
         <h3 class="results"><span><a class="two" href="#">Results</a></span></h3>
+
         <h3 class="results"><span><a class="two" href="https://2007.igem.org/Ljubljana/summary">Summary</a></span></h3>
         <ul>
         <ul>
-
           <li><a class="one" href="#">Flow Cytometry</a>&nbsp; </li>
+
 
-
           <li><a class="one" href="#">Luciferase Activity</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/achievements">Achievements</a>&nbsp; </li>
-
           <li><a class="one" href="#">Microscope</a>&nbsp; </li>
+
           <li><a class="one" href="https://2007.igem.org/Ljubljana/prospects">Prospects</a>&nbsp; </li>
 +
 
 +
            
         </ul>
         </ul>
       </div>
       </div>
<div id="ldiscussion">
<div id="ldiscussion">
-
         <h3 class="discussion"><span><a class="two" href="#">Discussion</a></span></h3>
+
         <h3 class="discussion"><span><a class="two" href="https://2007.igem.org/Ljubljana/team">Team</a></span></h3>
         <ul>
         <ul>
-
           <li><a class="one" href="#">Discussion (long)</a>&nbsp; </li>
+
            
 +
<br><br>
 +
<li><a class="one" href="https://2007.igem.org/Ljubljana/glossary">Terms & References</a>&nbsp; </li>
 +
<li><a class="one" href="https://2007.igem.org/Ljubljana/acknowledgements">Acknowledgements</a>&nbsp; </li>
         </ul>
         </ul>
       </div>
       </div>
-
 
+
<center>
-
<div id="lteam">
+
<a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2007.igem.org/Ljubljana" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2007.igem.org/Ljubljana" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onError="this.onError=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com'" />
-
        <h3 class="team"><span><a class="two" href="#">Team</a></span></h3>
+
</a>
-
        <ul>
+
</center>
-
          <li><a class="one" href="#">Students</a>&nbsp; </li>
+
-
          <li><a class="one" href="#">Supervisors</a>&nbsp; </li>
+
-
          <li><a class="one" href="#">Instructors</a>&nbsp; </li>
+
-
        </ul>
+
-
      </div>
+
-
 
+
     </div>
     </div>

Latest revision as of 16:29, 25 October 2007

Company Name

Synthetic biology provides the possibility to extend our defense against disease by employing our intelligence. In the spirit of synthetic biology we can combine different functional parts to assemble new cellular functions which are not yet existing in nature.

Abstract

HIV-1 virus is one of the most difficult targets for therapy because it hijacks our immune system and particularly because the virus mutates rapidly. This allows the viral armies to select the mutated variant strains which bypass the inhibitors that bind to specific residues on their targets. Current therapy consists of an inhibitor cocktail that decreases the probability of HIV to modify all target sequences at the same time.

We propose a different strategy, where we target a specific FUNCTION of virus, rather than any particular sequence. This viral function triggers a cellular response which can either employ antiviral defense or lead to a destruction of infected cells to prevent spread of the infection.

For implementation of this idea we have selected two functions: viral attachment to the cellular co-receptors CD4 and CCR5 and viral proprotein processing by its own protease. Binding of virus to T-cells leads to the formation of CD4-CCR5 heterodimer. In our device, formation of this heterodimer triggers reconstitution of a split protein (split ubiquitin or TEVP) which activates the specific proteolytic activity. This releases the T7 RNA polymerase from the membrane anchor and leads to transcription of the effector gene, which prevents the spread of viral infection.
The second implementation of this idea was to utilize the HIV-protease, which is required for viral maturation and cleaves a specific amino acid sequence. This target sequence was engineered between the membrane anchor and T7 polymerase. Activation of viral protease similarly as above releases the T7 polymerase and starts the defense program.

Important points of both approaches are to use the amplification of the signal, achieved by the use of T7 polymerase and the system's versatility, as we can select any number of different genes to be activated by the detection of viral function. The crucial aspect of our approach is that this system is not sensitive to viral mutations and is not activated only in case where the mutation leads to the loss of the viral function, which however also renders virus harmless at the same time.

We have tested many different constructs, contributing more than 70 new BioBricks and successfully demonstrated on mammalian cell culture activation of response gene by infection with HIV-1 pseudovirus.

PLEDGE: All experimental work on this project was performed from May to October 2007 by the undergraduate students participating in the team under the tutorial of instructors. All the students participated at iGEM for the first time.