McGill/Team 1: Fluorescence Complementation

From 2007.igem.org

(Difference between revisions)
(May 16)
(May 16)
Line 163: Line 163:
=== May 16 ===
=== May 16 ===
# Transformation of Cells
# Transformation of Cells
 +
## Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each)
 +
## Used 400uL of LB for each vial
 +
## Incubated for 1 hour in shaker at 37C, then incubate overnight at 37C
== June 2007 ==
== June 2007 ==

Revision as of 14:47, 17 May 2007

Home

Go to Team 2

May 2007
Su M Tu W Th F Sa
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
June 2007
Su M Tu W Th F Sa
      1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30



Contents

May 2007

May 14

  1. Made the same as May 15, but did not use Type 1 (milliQ) H2O, so the solutions were discarded.

May 15

  1. Made 1M CaCl2, and glycerol/CaCl2 solutions
    1. 1M CaCl2 Solution: 100mL was made
      1. CaCl2 Dihydrate = 146.986 g/mol
      2. 100mL of 1M CaCl2 requires 14.699g CaCl2 Dihydrate
    2. Glycerol/CaCl2 Solution: 30mL was made
      1. 5.00mL of 60% glycerol solution
      2. 3.00mL of 1M CaCl2
      3. 22.00mL of Type 1 (milliQ) H2O

May 16

  1. Transformation of Cells
    1. Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each)
    2. Used 400uL of LB for each vial
    3. Incubated for 1 hour in shaker at 37C, then incubate overnight at 37C

June 2007