McGill/Team 1: Fluorescence Complementation

From 2007.igem.org

(Difference between revisions)
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=== May 16 ===
=== May 16 ===
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# Transformation of Cells
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# Transformation of Cells:
## Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each)
## Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each)
## Used 400uL of LB for each vial
## Used 400uL of LB for each vial

Revision as of 14:48, 17 May 2007

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May 2007
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June 2007
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Contents

May 2007

May 14

  1. Made the same as May 15, but did not use Type 1 (milliQ) H2O, so the solutions were discarded.

May 15

  1. Made 1M CaCl2, and glycerol/CaCl2 solutions
    1. 1M CaCl2 Solution: 100mL was made
      1. CaCl2 Dihydrate = 146.986 g/mol
      2. 100mL of 1M CaCl2 requires 14.699g CaCl2 Dihydrate
    2. Glycerol/CaCl2 Solution: 30mL was made
      1. 5.00mL of 60% glycerol solution
      2. 3.00mL of 1M CaCl2
      3. 22.00mL of Type 1 (milliQ) H2O

May 16

  1. Transformation of Cells:
    1. Transformed 1 vial of cells with Jun vector, and another vial with Fos vector (1uL of vector each)
    2. Used 400uL of LB for each vial
    3. Incubated for 1 hour in shaker at 37C, then incubate overnight at 37C

June 2007