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Contents 1 May 2007 1.1 May 14 1.2 May 15 1.3 May 16 2 June 2007

May 2007

May 14

1. Made the same as May 15, but did not use Type 1 (milliQ) H2O, so the solutions were discarded.

May 15

1. Made 1M CaCl2, and glycerol/CaCl2 solutions
   1. 1M CaCl2 Solution: 100mL was made
      1. CaCl2 Dihydrate = 146.986 g/mol
      2. 100mL of 1M CaCl2 requires 14.699g CaCl2 Dihydrate
   2. Glycerol/CaCl2 Solution: 30mL was made
      1. 5.00mL of 60% glycerol solution
      2. 3.00mL of 1M CaCl2
      3. 22.00mL of Type 1 (milliQ) H2O

May 16

1. Discovered that the shaker was turned off sometime during the course of the night, and cells did not grow. Another possibility is that the cells have been stored for too long beforehand, and are not able to grow anymore.
2. Proceeded to seed cells again.

June 2007