From 2007.igem.org

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1/2YFP-Fos & 1/2YFPJun (exists?parts: BBa_J40004 & 40006) Based on PsfA promotor (new) from Dr Alan Grossman (M.I.T.)

This part is based on the paper “Autotransporters as Scaffolds for Novel Bacterial Adhesins: Surface proteins of Esherishia Coli Cells displaying Jun/Fos Dimerization Domains” By Estaban Veiga, Víctor de Lorenzo, and Luis Angel Fernández.

This part was to be based on the split YFP from McGill team 1 2006 but it was unavailable. We reasoned that by having two Ecoli lines that express one of the McGill components each when stimulated by the photodetection system we can get the two to adhere. FOS-Beta-YFPC BBa_J40004 JUN-Beta-YFPN BBa_J40006

Many potential problems here:

1. “complementation falls apart after a few hours at room temperature”
2. “PST sites present in construct-not a biobrick”
3. “not sure if complementation occurs when touching or simply close”
4. “expression fragile wipped out overnight”
5. “FOS part fluoresces without JUN part”
6. “sometimes cluster, not always!!!!”
7. “sometimes over whole single cell”

(1 to 3 see  : [[1]]) (4 to 7 see  : [[2]])

We originally considered a cadherin expressed on the surface but this opened up the question of how to get it there.