From 2007.igem.org
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| - | [[Melb:Background|<return to top of background>]] [[melbourne|<return to home page>]] [[Melb:And Gate |<next>]] | + | #redirect [[Melbourne/Blue Photosensor]] |
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| - | This part is based on “Photostimulation of a Sensory Rhodopsin II/HtrII/Tsr Fusion Chimera Activates CheA-Autophosphorylation and CheY-Phosphotransfer in Vitro” by Vishwa D. Trivedi and John L. Spudich, Biochemistry 2003, 42, 13887-13892.
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| - | Acording to this article the peak sensitivity is to 500+/-5nm, and results in a 3 fold activation of the Tsr.. CheA,W,Y connected system.
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| - | It is proposed to replace Tsr with homolgouse CopP.
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| - | SRII-HtrII fusion to which CopP is fused
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| - | CopA when phosphorylated by CopP is an activator for PsfA promoter sequence from
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| - | Dr Alan Grossman (M.I.T.)
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| - | Based on
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| - | *SRII-HtrII-Tsr fusion from Prof J.L. Spudich (university of Texas)[[melb:spudich N sequence]]
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| - | *BBa_J51000 (ComP) kinase
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| - | *BBa_J51001 (ComA) activator
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| - | PARTS:
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| - | * SrfA promoter
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| - | * ComA protein generator
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| - | * SRII-ComP photosensor
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| - | * Any phyco construction genes?
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| - | SRII is from Natronomonas pharaonis.
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| - | Tsr fusion was made by Jung et al J Bacteriol 183 6365-6371 (2001) they propose a mechanism.
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| - | I don’t see why anyone thinks this will work!!! Currently a conformational change induced by light increases affinity in TSR for Che family which leads to cross phosphorylation. To replace TSR with a kinase would require the kinase activity to be modulated – hence matching using homology as was done for tsr is not likely to work.
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| - | Also what are the normal functions of ComP etc.
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Latest revision as of 09:55, 6 August 2007
- redirect Melbourne/Blue Photosensor
