Melb:Primary

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Revision as of 08:06, 1 July 2007 by PhillipDodson (Talk | contribs)

Contents

Primary Reagents & Disposables

Primary Reagents

Kits

  • Miniprep kit
  • Gel clean-up system

Enzymes

  • Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
  • Ligase
  • Competent cells (both for plasmid purification and protein expression)
  • DNA standard

Chemicals

  • Ingredients for media (LB, SOB)
  • Glycerol
  • Buffers (including TAE and TE)
  • Loading dye for DNA gel

Disposables

TUBES:

  • Falcon: 10ml, 50ml
  • microcentrifuge: 1.7ml

Tips

  • Tips (10, 200, 1000 ul)

Other

  • Gloves (Large , Med, Small)
  • Scalpels
  • Tube racks
  • Freezer boxes
  • Parafilm