Melbourne/GvpL site dirrected primers
From 2007.igem.org
(Difference between revisions)
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| - | Oligonucleotide information: | + | Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html |
{|border="1" | {|border="1" | ||
| - | !Primer Name !!Length (nt.) !!Tm !!Duplex Energy at 68°C !!Energy Cost of Mismatches | + | !Primer Name !!Length (nt.) !!Tm !!Duplex Energy at 68°C !!Energy Cost of Mismatches !!GC content !!Tm ver!!OD=1 |
|- | |- | ||
| - | |g213a ||39 ||78.45°C ||-44.89 kcal/mole ||6.5% | + | |g213a ||39 ||78.45°C ||-44.89 kcal/mole ||6.5% || 41% || 64 ||2216nM |
|- | |- | ||
| - | |g213a_antisense ||39 ||78.45°C ||-42.10 kcal/mole|| 7.8% | + | |g213a_antisense ||39 ||78.45°C ||-42.10 kcal/mole||7.8% || 41% || 64 ||2216nM |
|- | |- | ||
| - | |g318a ||47 ||78.97°C ||-46.02 kcal/mole ||6.3% | + | |g318a ||47 ||78.97°C ||-46.02 kcal/mole || 6.3%|| 7.8% || 65 || 1754nM|| |
|- | |- | ||
| - | |g318a_antisense ||47 ||78.97°C ||-42.40 kcal/mole ||7.7% | + | |g318a_antisense ||47 ||78.97°C ||-42.40 kcal/mole ||7.7% || 7.8% || 65 || 1754nM|| |
|- | |- | ||
| - | |g351a ||46 ||78.02°C ||-43.40 kcal/mole ||5.3% | + | |g351a ||46 ||78.02°C ||-43.40 kcal/mole ||5.3% || 33% || 64||1820nM |
|- | |- | ||
| - | |g351a_antisense ||46 ||78.02°C ||-40.27 kcal/mole ||7.4% | + | |g351a_antisense ||46 ||78.02°C ||-40.27 kcal/mole ||7.4%|| 33% || 64||1820nM |
|- | |- | ||
| - | |g696a ||39 ||78.45°C ||-42.46 kcal/mole ||5.4% | + | |g696a ||39 ||78.45°C ||-42.46 kcal/mole ||5.4%|| 41% ||64||2496nM |
|- | |- | ||
| - | |g696a_antisense ||39 ||78.45°C ||-43.97 kcal/mole ||6.8% | + | |g696a_antisense ||39 ||78.45°C ||-43.97 kcal/mole ||6.8% || 41% ||64||2496nM |
|} | |} | ||
Revision as of 02:31, 17 July 2007
Primer sequences: Primer Name Primer Sequence (5' to 3')
| g213a | 5'-aggatatgagctggctgcaagaaaaagcatttcatcatc-3' |
| g213a_antisense | 5'-gatgatgaaatgctttttcttgcagccagctcatatcct-3' |
| g318a | 5'-ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag-3' |
| g318a_antisense | 5'-ctttgttaatctcaatagctgcttgcagactttcttcacctttataa-3' |
| g351a | 5'-attgagattaacaaagaaaagatagaaaattcactgacgctgcttc-3' |
| g351a_antisense | 5'-gaagcagcgtcagtgaattttctatcttttctttgttaatctcaat-3' |
| g696a | 5'-ttcttctcccgtctctgcaaattactaagttcgtaaacg-3' |
| g696a_antisense | 5'-cgtttacgaacttagtaatttgcagagacgggagaagaa-3' |
Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html
| Primer Name | Length (nt.) | Tm | Duplex Energy at 68°C | Energy Cost of Mismatches | GC content | Tm ver | OD=1 | |
|---|---|---|---|---|---|---|---|---|
| g213a | 39 | 78.45°C | -44.89 kcal/mole | 6.5% | 41% | 64 | 2216nM | |
| g213a_antisense | 39 | 78.45°C | -42.10 kcal/mole | 7.8% | 41% | 64 | 2216nM | |
| g318a | 47 | 78.97°C | -46.02 kcal/mole | 6.3% | 7.8% | 65 | 1754nM | |
| g318a_antisense | 47 | 78.97°C | -42.40 kcal/mole | 7.7% | 7.8% | 65 | 1754nM | |
| g351a | 46 | 78.02°C | -43.40 kcal/mole | 5.3% | 33% | 64 | 1820nM | |
| g351a_antisense | 46 | 78.02°C | -40.27 kcal/mole | 7.4% | 33% | 64 | 1820nM | |
| g696a | 39 | 78.45°C | -42.46 kcal/mole | 5.4% | 41% | 64 | 2496nM | |
| g696a_antisense | 39 | 78.45°C | -43.97 kcal/mole | 6.8% | 41% | 64 | 2496nM |
