Melbourne/GvpL site dirrected primers

From 2007.igem.org

(Difference between revisions)
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Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html
Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html
{|border="1"
{|border="1"
-
!Primer Name !!Length (nt.) !!Tm !!Duplex Energy at 68°C !!Energy Cost of Mismatches !!GC content !!Tm ver!!OD=1
+
!Primer Name !!Length (nt.) !!Tm !!Duplex Energy at 68°C !!Energy Cost of Mismatches !!GC content !!Tm ver!!OD=1!!bp before!!bp after
|-
|-
-
|g213a ||39 ||78.45°C ||-44.89 kcal/mole ||6.5% || 41%  || 64 ||2216nM
+
|g213a ||39 ||78.45°C ||-44.89 kcal/mole ||6.5% || 41%  || 81 deg C ||2216nM||19||19
|-
|-
-
|g213a_antisense ||39 ||78.45°C ||-42.10 kcal/mole||7.8% || 41%  || 64 ||2216nM
+
|g213a_antisense ||39 ||78.45°C ||-42.10 kcal/mole||7.8% || 41%  || 32% ||2216nM|| ||
|-
|-
-
|g318a ||47 ||78.97°C ||-46.02 kcal/mole || 6.3%|| 7.8% || 65 || 1754nM||
+
|g318a ||47 ||78.97°C ||-46.02 kcal/mole || 6.3%|| 30%|| 80 deg C || 1754nM||25||21
|-
|-
-
|g318a_antisense ||47 ||78.97°C ||-42.40 kcal/mole ||7.7%  || 7.8% || 65 || 1754nM||
+
|g318a_antisense ||47 ||78.97°C ||-42.40 kcal/mole ||7.7%  || 30% || 80 deg C || 1754nM|| ||
|-
|-
-
|g351a ||46 ||78.02°C ||-43.40 kcal/mole ||5.3% || 33% || 64||1820nM
+
|g351a ||46 ||78.02°C ||-43.40 kcal/mole ||5.3% ||     33% || 80 deg C||1820nM||26||19
|-
|-
-
|g351a_antisense ||46 ||78.02°C ||-40.27 kcal/mole ||7.4%||  33% || 64||1820nM
+
|g351a_antisense ||46 ||78.02°C ||-40.27 kcal/mole ||7.4%||  33% || 80 deg C||1820nM||  ||
|-  
|-  
-
|g696a ||39 ||78.45°C ||-42.46 kcal/mole ||5.4%||  41% ||64||2496nM
+
|g696a ||39 ||78.45°C ||-42.46 kcal/mole ||5.4%||  41% ||81||2496nM||19||19
|-  
|-  
-
|g696a_antisense ||39 ||78.45°C ||-43.97 kcal/mole ||6.8% ||  41% ||64||2496nM
+
|g696a_antisense ||39 ||78.45°C ||-43.97 kcal/mole ||6.8% ||  41% ||81 Deg C||2496nM|| ||
|}
|}

Revision as of 02:25, 18 July 2007

Primer sequences: Primer Name Primer Sequence (5' to 3')

g213a 5'-aggatatgagctggctgcaagaaaaagcatttcatcatc-3'
g213a_antisense 5'-gatgatgaaatgctttttcttgcagccagctcatatcct-3'
g318a 5'-ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag-3'
g318a_antisense 5'-ctttgttaatctcaatagctgcttgcagactttcttcacctttataa-3'
g351a 5'-attgagattaacaaagaaaagatagaaaattcactgacgctgcttc-3'
g351a_antisense 5'-gaagcagcgtcagtgaattttctatcttttctttgttaatctcaat-3'
g696a 5'-ttcttctcccgtctctgcaaattactaagttcgtaaacg-3'
g696a_antisense 5'-cgtttacgaacttagtaatttgcagagacgggagaagaa-3'

Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html

Primer Name Length (nt.) Tm Duplex Energy at 68°C Energy Cost of Mismatches GC content Tm verOD=1bp beforebp after
g213a 39 78.45°C -44.89 kcal/mole 6.5% 41% 81 deg C 2216nM1919
g213a_antisense 39 78.45°C -42.10 kcal/mole7.8% 41% 32% 2216nM
g318a 47 78.97°C -46.02 kcal/mole 6.3% 30% 80 deg C 1754nM2521
g318a_antisense 47 78.97°C -42.40 kcal/mole 7.7% 30% 80 deg C 1754nM
g351a 46 78.02°C -43.40 kcal/mole 5.3% 33% 80 deg C1820nM2619
g351a_antisense 46 78.02°C -40.27 kcal/mole 7.4% 33% 80 deg C1820nM
g696a 39 78.45°C -42.46 kcal/mole 5.4% 41% 812496nM1919
g696a_antisense 39 78.45°C -43.97 kcal/mole 6.8% 41% 81 Deg C2496nM
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