Melbourne/GvpL site dirrected primers

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Primer sequences: Primer Name Primer Sequence (5' to 3')

g213a 5'-aggatatgagctggctgcaagaaaaagcatttcatcatc-3'
g213a_antisense 5'-gatgatgaaatgctttttcttgcagccagctcatatcct-3'
g318a 5'-ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag-3'
g318a_antisense 5'-ctttgttaatctcaatagctgcttgcagactttcttcacctttataa-3'
g351a 5'-attgagattaacaaagaaaagatagaaaattcactgacgctgcttc-3'
g351a_antisense 5'-gaagcagcgtcagtgaattttctatcttttctttgttaatctcaat-3'
g696a 5'-ttcttctcccgtctctgcaaattactaagttcgtaaacg-3'
g696a_antisense 5'-cgtttacgaacttagtaatttgcagagacgggagaagaa-3'

Oligonucleotide information:

Primer Name Length (nt.) Tm Duplex Energy at 68°C Energy Cost of Mismatches
g213a 39 78.45°C -44.89 kcal/mole 6.5%
g213a_antisense 39 78.45°C -42.10 kcal/mole 7.8%
g318a 47 78.97°C -46.02 kcal/mole 6.3%
g318a_antisense 47 78.97°C -42.40 kcal/mole 7.7%
g351a 46 78.02°C -43.40 kcal/mole 5.3%
g351a_antisense 46 78.02°C -40.27 kcal/mole 7.4%
g696a 39 78.45°C -42.46 kcal/mole 5.4%
g696a_antisense 39 78.45°C -43.97 kcal/mole 6.8%