Melbourne/GvpL site dirrected primers
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Primer sequences: Primer Name Primer Sequence (5' to 3')
g213a | 5'-aggatatgagctggctgcaagaaaaagcatttcatcatc-3' |
g213a_antisense | 5'-gatgatgaaatgctttttcttgcagccagctcatatcct-3' |
g318a | 5'-ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag-3' |
g318a_antisense | 5'-ctttgttaatctcaatagctgcttgcagactttcttcacctttataa-3' |
g351a | 5'-attgagattaacaaagaaaagatagaaaattcactgacgctgcttc-3' |
g351a_antisense | 5'-gaagcagcgtcagtgaattttctatcttttctttgttaatctcaat-3' |
g696a | 5'-ttcttctcccgtctctgcaaattactaagttcgtaaacg-3' |
g696a_antisense | 5'-cgtttacgaacttagtaatttgcagagacgggagaagaa-3' |
Oligonucleotide information:
Primer Name | Length (nt.) | Tm | Duplex Energy at 68°C | Energy Cost of Mismatches |
---|---|---|---|---|
g213a | 39 | 78.45°C | -44.89 kcal/mole | 6.5% |
g213a_antisense | 39 | 78.45°C | -42.10 kcal/mole | 7.8% |
g318a | 47 | 78.97°C | -46.02 kcal/mole | 6.3% |
g318a_antisense | 47 | 78.97°C | -42.40 kcal/mole | 7.7% |
g351a | 46 | 78.02°C | -43.40 kcal/mole | 5.3% |
g351a_antisense | 46 | 78.02°C | -40.27 kcal/mole | 7.4% |
g696a | 39 | 78.45°C | -42.46 kcal/mole | 5.4% |
g696a_antisense | 39 | 78.45°C | -43.97 kcal/mole | 6.8% |