Melbourne/Lab GV Notebook

From 2007.igem.org

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[[Melbourne/Lab Notebook|<Return to Lab notebook>]]  [[Melbourne|  <team home page>]]
[[Melbourne/Lab Notebook|<Return to Lab notebook>]]  [[Melbourne|  <team home page>]]
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*Image:Image-melbourne-Development path gv.jpg|850px|Development of BBa_I750016]]
*[[Melbourne/Lab Notebook gv 0|Synopsis]]
*[[Melbourne/Lab Notebook gv 0|Synopsis]]
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=Steps:=
*[[Melbourne/Lab Notebook gv 1|Create Glycerol stocks of gas vesicle plasmids and cells supplied by Maura Cannon]]
*[[Melbourne/Lab Notebook gv 1|Create Glycerol stocks of gas vesicle plasmids and cells supplied by Maura Cannon]]
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*[[Melbourne/Lab Notebook gv 2|First round of site directed mutagenesis]]
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*Site dirrected mutagenesis:
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*[[Melbourne/Lab Notebook gv 3|Second round of site directed mutagenesis]]
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**[[Melbourne/Lab Notebook gv 2|Round 1]]
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*[[Melbourne/Lab Notebook gv 4|Third round of site directed mutagenesis]]
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**[[Melbourne/Lab Notebook gv 3|Round 2]]
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*[[Melbourne/Lab Notebook gv 5|Fourth round of site directed mutagenesis]]
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**[[Melbourne/Lab Notebook gv 4|Round 3]]
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**[[Melbourne/Lab Notebook gv 5|Round 4]]
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*[[Melbourne/Lab Notebook gv 6|First attempt at PCR insertion of biobrick prefix and suffix]]
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*PCR based insertion of Biobrick prefix and suffix, digestion and ligation to BBa_J61035
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*[[Melbourne/Lab Notebook gv 7|Second attempt]]
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**[[Melbourne/Lab Notebook gv 6|attempt 1]]
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*[[Melbourne/Lab Notebook gv 8|Third attempt]]
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**[[Melbourne/Lab Notebook gv 7|attempt 2]]
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*[[Melbourne/Lab Notebook gv 9|Fourth attempt]]
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**[[Melbourne/Lab Notebook gv 8|attempt 3]]
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**[[Melbourne/Lab Notebook gv 9|attempt 4]]
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*Testing Floatation
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**[[Melbourne/Lab Notebook gv F1|attempt 1]]
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**[[Melbourne/Lab Notebook gv F2|pNL29 in DH5a and 41C in XL10-Gold]]
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**[[Melbourne/Lab Notebook gv F3|pNL29 and 41C,42A,42B,43A,43B,44A,44B all in DH5a]]
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*Sequencing
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**[[Melbourne/Lab Notebook gv seq64A|seq64A]]
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**[[Melbourne/Lab Notebook gv seq42A|seq42A]]
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**[[Melbourne/Lab Notebook gv seq42B|seq42B]]
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**[[Melbourne/Lab Notebook gv seq43A|seq43A]]
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**[[Melbourne/Lab Notebook gv seq43B|seq43B]]
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**[[Melbourne/Lab Notebook gv seq44A|seq44A]]
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**[[Melbourne/Lab Notebook gv seq44B|seq44B]]
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*Future directions

Revision as of 07:48, 25 October 2007

<Return to Lab notebook> <team home page>

  • Image:Image-melbourne-Development path gv.jpg|850px|Development of BBa_I750016]]

Steps:

Retrieved from "http://2007.igem.org/wiki/index.php/Melbourne/Lab_GV_Notebook"