Melbourne/Lab Notebook

From 2007.igem.org

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==Week 1==
==Week 1==
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*25 June 2007:  
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*25 June 2007: Prepared LB agar plates Amp & Kana.
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**Prepared LB agar plates Amp & Kana.
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*25 June 2007: [[Melbourne/IGEM 2007 kit|resuspended the following from Registry plates]] & [[Melbourne/Transformation Protocol shorter|Transformed (shorter protocol)]] into competent DH5alpha cells.
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**[[Melbourne/IGEM2007 kit|resuspended the following from Registry plates]] & [[Melbourne/Transformation Protocol shorter|Transformed (shorter protocol)]] into competent DH5alpha cells.
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*#[[Melbourne/BBa_I15008|'''P2 21A''']] - (BBa_I15008, ho1, Kan) -> No colonies on plates
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**#[[Melbourne/BBa_I15008|'''P2 21A''']] - (BBa_I15008, ho1, Kan) -> No colonies on plates
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*#[[Melbourne/BBa_I15009|'''P2 21C''']] - (BBa_I15009, PcyA, Kan) ->No colonies on plates
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**#[[Melbourne/BBa_I15009|'''P2 21C''']] - (BBa_I15009, PcyA, Kan) ->No colonies on plates
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*#[[Melbourne/BBa_R0084|'''P1 11H''']] - (BBa_R0084, OmpR positive promoter, Amp)->Small number of colonies.
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**#[[Melbourne/BBa_R0084|'''P1 11H''']] - (BBa_R0084, OmpR positive promoter, Amp)->Small number of colonies.
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*26 June 2007
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*26 June 2007: [[Melbourne/IGEM 2007 kit|resuspended the following from Registry plates]] & [[Melb:Transformation Protocol|Transformed]] into Joe's competent DH5alpha cells.
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**[[Melbourne/IGEM2007 kit|resuspended the following from Registry plates]] & [[Melb:Transformation Protocol|Transformed]] into Joe's competent DH5alpha cells.
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*#[[Melbourne/BBa_B0034|'''P1 3O''']] (BBa_B0034, RBS, Amp)
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**#[[Melbourne/BBa_B0034|'''P1 3O''']] (BBa_B0034, RBS, Amp)
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*#[[Melbourne/BBa_C0051|'''P1 5G''']] (BBa_C0051, c1 protein, Amp)
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**#[[Melbourne/BBa_C0051|'''P1 5G''']] (BBa_C0051, c1 protein, Amp)
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*#[[Melbourne/BBa_B0010|'''P2 3P''']] (BBa_B0010, Terminator, Amp)
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**#[[Melbourne/BBa_B0010|'''P2 3P''']] (BBa_B0010, Terminator, Amp)
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**Streaked the following cells:
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*26 June 2007: Streaked the following cells:
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**#[[Melbourne/pJS010|'''pJS010''']] (from solid agar, Amp)
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*#[[Melbourne/pJS010|'''pJS010''']] (from solid agar, Amp)
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**#[[Melbourne/Fusion|'''Fusion protein''']] (from glycerol stock, Amp?)
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*#[[Melbourne/Fusion|'''Fusion protein''']] (from glycerol stock, Amp?)
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**#[[Melbourne/BBa_I15010|'''BBa_I15010''']] (from solid agar, Kan)
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*#[[Melbourne/BBa_I15010|'''BBa_I15010''']] (from solid agar, Kan)
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*27 June 2007
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*27 June 2007: [[Melb:Transformation Protocol|Transformed]] into Joe's competent DH5alpha cells.
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**[[Melb:Transformation Protocol|Transformed]] into Joe's competent DH5alpha cells.
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*#[[Melbourne/BBa_I15008|'''P2 21A''']] (Kan)
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##[[Melbourne/BBa_I15008|'''P2 21A''']] (Kan)
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*#[[Melbourne/BBa_I15009|'''P2 21C''']] (Kan)
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##[[Melbourne/BBa_I15009|'''P2 21C''']] (Kan)
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Revision as of 06:11, 5 July 2007

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Contents

Week 1


  • 26 June 2007: Streaked the following cells:
    1. pJS010 (from solid agar, Amp)
    2. Fusion protein (from glycerol stock, Amp?)
    3. BBa_I15010 (from solid agar, Kan)


Liquid culture

  1. Prepared 30mL of Amp LB (100ug/mL). Prepared 5mL Kan LB (50mg/mL).
  2. Aliquoted 5mL Amp LB into 6 50mL falcon tubes
  3. To the Amp LB aliquots single transformed colonies from plates of the following were introduced:
  4. To the Kan LB a single colony from the transformation plate of BBa_I15010 was introduced.
  5. Cells incubated at 37degrees with shaking overnight.

28 June 2007

Miniprep

  1. Minipreped the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks:
  2. Stored in -20 freezer

Digest

Liquid culture

  1. Cultured the following cells from transformed plates:

29 June 2007

Miniprep

  1. Minipreped the cultures grown overnight after putting aside 1mL(sterile) for glycerol stocks:
  1. Stored in -20 freezer

30 June 2007

Week 2

2 July 2007

3 July 2007

4 July 2007

Ampicillin Plates

  • Made up 25 Ampicillin plates using 400mL LB agar prepared on the 25th of June.
    • Used 100mg/ml Ampicillin stock from Gooley Lab - In bag in left freezer near our lab bench.

Tranformation

Transformed the following and grew on new ampicillin plates

  • P1 5H
  • P4 8J
  • P2 15L
    • Also Plated an untransformed control (subjected to transformation protocol in absense of DNA) and a blank plate control (no cells)

Liquid Culture

5 July 2007

Miniprep

6 July 2007

Week 3

9 July 2007

10 July 2007

11 July 2007

12 July 2007

13 July 2007

Week 4

16 July 2007

17 July 2007

18 July 2007

19 July 2007

20 July 2007

Week 5

23 July 2007

24 July 2007

25 July 2007

26 July 2007

27 July 2007

Week 6

30 July 2007

31 July 2007

1 Aug 2007

2 Aug 2007

3 Aug 2007