Melbourne/Primary Reagents & disposables

From 2007.igem.org

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===Enzymes===
===Enzymes===
-
*Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)
+
*[[Melbourne/primary Restriction enzymes]]
-
*Ligase
+
** NotI,  
-
*Competent cells (both for plasmid purification and protein expression)
+
**EcoRI,  
-
*DNA standard
+
**XbaI,
 +
**HindIII,
 +
**SpeI,
 +
*[[Melbourne/primary Ligase|Ligase]]
 +
*[[Melbourne/primary competant DH5a|DH5a Competent cells]]
 +
*[[Melbourne/primary DNA marker|DNA ladder]]
===Chemicals===
===Chemicals===
-
*Ingredients for media (LB, SOB)
+
*[[Melbourne/primary agar|Agar]]
-
*Glycerol
+
*[[Melbourne/primary Agarose|Agarose]]
-
*Buffers (including TAE and TE)
+
*[[Melbourne/primary EB|Ethidium Bromide]]
-
*Loading dye for DNA gel
+
*[[Melbourne/primary EDTA|EDTA]]*[[Melbourne/primary glycerol|Glycerol]]
 +
*[[Melbourne/primary dna loading|Loading dye for DNA gel]]
 +
*[[Melbourne/primary Nacl|NaCl]]
 +
*[[Melbourne/primary Tris|TRIS]]
 +
===Other===
 +
*[[Melbourne/primary ice|ice]]
 +
*[[Melbourne/primary milliq|milliQ water]]
 +
*[[Melbourne/primary distilled|Distilled water]]
==Disposables==
==Disposables==
 +
*[[Melbourne/primary Parrafilm|Parrafilm]]
===TUBES:===
===TUBES:===
-
*Falcon: 10ml, 50ml
+
*[[Melbourne/primary falcon|Falcon: 10ml, 50ml]]
*microcentrifuge: 1.7ml
*microcentrifuge: 1.7ml
*PCR
*PCR

Revision as of 04:51, 4 July 2007

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Contents

Primary Reagents

Kits

  • Miniprep kit
  • Gel clean-up system
  • Site directed mutagenesis (stratagene)
    • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
    • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
    • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
  • Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Enzymes

Chemicals

Other

Disposables

TUBES:

Tips

  • Tips (10, 200, 1000 ul)

Other

  • Gloves (Large , Med, Small)
  • Scalpels
  • Tube racks
  • Freezer boxes
  • Parafilm