Melbourne/Primary Reagents & disposables

From 2007.igem.org

(Difference between revisions)
Line 25: Line 25:
*[[Melbourne/primary Agarose|Agarose]]
*[[Melbourne/primary Agarose|Agarose]]
*[[Melbourne/primary EB|Ethidium Bromide]]
*[[Melbourne/primary EB|Ethidium Bromide]]
-
*[[Melbourne/primary EDTA|EDTA]]*[[Melbourne/primary glycerol|Glycerol]]
+
*[[Melbourne/primary EDTA|EDTA]]
 +
*[[Melbourne/primary glycerol|Glycerol]]
*[[Melbourne/primary dna loading|Loading dye for DNA gel]]
*[[Melbourne/primary dna loading|Loading dye for DNA gel]]
*[[Melbourne/primary Nacl|NaCl]]
*[[Melbourne/primary Nacl|NaCl]]

Revision as of 05:53, 4 July 2007

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Contents

Primary Reagents

Kits

  • Miniprep kit
  • Gel clean-up system
  • Site directed mutagenesis (stratagene)
    • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
    • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
    • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
  • Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Enzymes

Chemicals

Other

Disposables

TUBES: