Melbourne/cannon plasmid pNL26 res map
From 2007.igem.org
(Difference between revisions)
Revision as of 23:01, 12 July 2007
Restriction map of core enzymes from: http://arbl.cvmbs.colostate.edu/molkit/mapper/index.html
| Enzyme | Cuts | --------- Base 5' to Cleave Sites ---------- |
|---|---|---|
| BamHI | 2 | 2916 , 5033 |
| BglII | 1 | 8438 |
| ClaI | 5 | 7304 , 7340 , 7698 , 9196 , 10004 |
| EcoRI | 2 | 5789 , 10026 |
| EcoRV | 2 | 9273 , 9491 |
| HincII | 3 | 3652 , 4927 , 9757 |
| HindIII | 3 | 3469 , 4121 , 8973 |
| KpnI | 2 | 25 , 3408 |
| NcoI | 2 | 5296 , 5365 |
| NotI | 1 | 2897 |
| PstI | 7 | 2937 , 5453 , 5831 , 5936 , 9073 , 9106 , 9326 |
| SacI | 2 | 2884 , 7910 |
| SalI | 0 | |
| SmaI | 1 | 2924 |
| XbaI | 1 | 2904 |
| XhoI | 2 | 6 , 5345 |
