Melbourne/cannon plasmid pNL26 res map

From 2007.igem.org

< Melbourne(Difference between revisions)
 
Line 17: Line 17:
|HincII        ||4      ||17  3662  4937  9767
|HincII        ||4      ||17  3662  4937  9767
|-
|-
-
|HindIII        ||3     ||3479  4131  8983
+
|HindIII        ||4     ||3479  4131  8983 (and 0 for circular)
|-
|-
|KpnI          ||2        || 40  3418
|KpnI          ||2        || 40  3418

Latest revision as of 06:05, 13 July 2007

Restriction map of core enzymes from: http://arbl.cvmbs.colostate.edu/molkit/mapper/index.html

Enzyme Cuts --------- Base 5' to Cleave Sites ----------
BamHI 2 2931 5043
BglII 1 8448
ClaI 6 7 7314 7350 7708 9206 10014
EcoRI 2 5799 10036
EcoRV 2 9283 9501
HincII 4 17 3662 4937 9767
HindIII 4 3479 4131 8983 (and 0 for circular)
KpnI 2 40 3418
NcoI 2 5306 5375
NotI 1 2912
PstI 7 2947 5463 5841 5946 9083 9116 9336
SacI 2 2899 7920
SalI 1 15
SmaI 1 2939
XbaI 1 2919
XhoI 2 21 5355