Melbourne/meeting 14 September 2007
From 2007.igem.org
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| - | |1 | + | |1 October|| Jamboree attendance fees due $100USD per team member. |
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| - | |26 October|| Project and part documentation | + | |26 October|| Project and wiki & part documentation frozen, DNA received USA. |
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Latest revision as of 02:50, 20 September 2007
Contents |
5:30 Action points from last meeting:
- Jan: Plan for blue light on wiki by 17th August- Done but needs more work: couldn’t get anyone else to implement it based on this!
5:35 Status Reports: (5 minutes each including any discussion)
- Pat : Red light receptor progress/design/plan- needs?
- M30109: Must construct from scratch plan to be produced by Pat.
- This is then drives RBS-ComP-term
- Also required is ConstitutiveProm-RBS-ComA-term
- Jan & Alisa: Blue light receptor design/plan progress - needs?
- Primers ComA and Com P all in
- Rapid Ligase 5min purchased
- ComP and SopII to be conbined at variouse points by using 4 primer stiching:
- Comp P forward
- SopII reverse
- ComP-SoPII overlap sense
- ComP-SopII overlap antisense
- Note that there are 7 different stiching pairs that result in combination at different points.
- The product is then combined with RBS
- Then transcription terminator
- The constitutive promotor to create protein generator part.
- Also Required is reporter construct PsfPromoter-RBS-LacZ-term
- Phil: Gas vesicles progress/design/plan - needs?
- Primers for part creation in
- No Progress , round 4 of mutagenesis plated awaits culture and diggest(4 plates).
- Buoyancy experiment needs repeating
- Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
- Pat to provide these.
5.55 New Agenda Items:
- Phil: Administrative Timetable: (5 Weeks to go)
- Chris: Next years Comp Plan/preparations - no progress
- Next meeting: <b/> 21th September (Friday) 5.30PM.
6:15 End
Igem Administrative Timetable:
| 1 October | Final team rosters due. |
| 1 October | Jamboree attendance fees due $100USD per team member. |
| 26 October | Project and wiki & part documentation frozen, DNA received USA. |
| 3-4 November | iGEM Competition Jamboree, MIT, USA. |
.
- re prism :Edmund optics:VIS60 Schott veril linear variable interference filter SGD$1600 25mm wide 60mm long linear 400nm-700nm (contact sheet)
N47-279 eqilateral prism 35mm long 35mm face SGD$162
Documentation wiki status:
- Priority should be on plans and experimental report analysis and conclusions-----Alisa .
- If only Pat & Alisa doing lab work not much documentation needed.
- Equipement:
- MissingLas3000 method (in lab notebook)
- Missing DNA measurement protocol (eppendorf)
- Reagents pages incomplete:
- Some location pictures not finished or uploaded.
- Some newer reagents not entered.
- Secondary reagents pages not made:
- LB, Plates, TAE , NZY+ Broth
- Protocol part:
- No connections from Protocols to reagents.
- Ligation protocol missing
- Lab notebook:
- Format uses too many lines to see connections between days.
- Latter part missing links
- Can table of contents be restricted to date level?
- Experimental reports:
- Lacks discussion/conclusion
- Ligation experiment missing
- Lattest few gells not marked up and entered.
- Project overview link broken
- Background:
- GFP missing ,
- Red photo sensor to GFP test harness missing.
- Plan: Only Gas vesicles plan entered.
| Individual | Desired time committment |
|---|---|
| Pat | About 4h/wk Monday afternoon or Tuesday morning |
| Alisa | About 4h/wk - Thursday afternoon |
| Jan | About 10h/pw - flexible weekday mornings |
| Phil | About 18h/pw - Thursday morning, Friday, Saturday or Sunday, |
