Melbourne/meeting 27 September 2007

From 2007.igem.org

(Difference between revisions)

Revision as of 04:00, 27 September 2007

Contents

3:30 Action points from last meeting:

  • Alisa: Get plan for blue light on wiki (from 10 Aug). Alisa has done a plan but this is not on Wiki.

3:35 Status Reports: (5 minutes each including any discussion)

  • Pat : Red light receptor progress/design/plan- needs?
    • M30109: Must construct from scratch plan to be produced by Pat.
      • craig seqenced Cph8 from M30109 first part present
      • Cph8 from initial plate not functional
    • This is then drives RBS-ComP-term
      • RBS-ComP -done
    • Also required is ConstitutiveProm-RBS-ComA-term
      • RBS-ComA-term -done


  • Jan & Alisa: Blue light receptor design/plan progress - needs?
    • ComP and SopII conbined at variouse points by using 4 primer stiching:
      • Comp P forward
      • SopII reverse
      • ComP-SoPII overlap sense
      • ComP-SopII overlap antisense
    • Note that there are 7 different stiching pairs that result in combination at different points.-Done
    • The product is then combined with RBS -Done
    • Sequencing being performed Friday
    • Then transcription terminator
    • The constitutive promotor to create protein generator part.
    • Also Required is reporter construct PsfPromoter-RBS-LacZ-term
    • Only three colonies pSFR8 grew in LB
  • Phil: Gas vesicles progress/design/plan - needs?
    • Ligation P48J (SpeI & PstI digested) to PCR(GvpL) underway
    • Ligation P48J (SpeI & PstI digested) to PCR(GvpB to GvpU inclusive)(XbaI and PstI digested) underway
    • Buoyancy experiment needs repeating
    • Other parts required from kit->glycerol stocks: B034 (P1-3O) , B0015 (P1-1I or P3-3O), J61034 (P4-8F or 8H)
    • Pat to provide these.

3.55 New Agenda Items:

  • Sally Grass (chemical and biochemical engineering):
    • interested in signal transduction.
    • moving to lab in bio21.
    • will help promote next years comp
    • Suggested Chris contact Lisa Hayes in chem eng re promotion.
  • Phil: Administrative Timetable: (4 Weeks to go)
  • Chris: Next years Comp Plan/preparations
    • Slide prepared
  • Alisa: To make competent cells to replace Joes
  • Alisa: Tshirt should be simple based on Melb Uni logo, will propose a design
  • Paul: suggested Ian Potter foundation may provide travel funding
  • Next meeting: <b/> 27th September (Thurs) 3.30PM.

5:00 End