Melbourne/primary DNA marker

From 2007.igem.org

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Contents

Physical data

  • Description:1Kb Plus DNA Ladder, 1ug/ml , 250ml , Store at -20 deg C
  • apply 0.1ug of ladder per mm of lane width.
  • Recomend BlueJuice loading buffer at 2x.

Method

  • Recieved ladder split into twelve 20uL alliquots and stored in ependorfs in Igem Freezer Box 1.
  • To one alliquot 400uL of 1x Green Loading dye is added which turns it Blue.
  • Place 20uL of this solution into the 10mm well. (1ug/well)(0.1ug/mm of well)
  • link to user datasheet/manuals:
  • Approximate cost: $0.00 AUD/mg

Lab Location

  • Location
  • Physical apearance:
  • Permision to use required? No

Store

  • Available from Bio21 store? YES
  • Order number in store:
  • Order QTY:

Supplier:

  • Supplier:
  • Suppliers part no:
  • Contact details for supplier:
  • Email:
  • Phone:

Manufacturer:

  • Manufacturer: Invitrogen
  • Manufacturers Part No: Cat no. 10787-018
  • Contact details for Manufacturer: www.invitrogen.com
  • Link to Manual or copy of Manufacturers manual
  • Email:
  • Phone:



  • PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007