NYMU Taipei/Lab Notes/2007 10 3

From 2007.igem.org

(Difference between revisions)
(GEL check for digestions)
(System 1)
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==GEL check for digestions==
==GEL check for digestions==
=== System 1 ===
=== System 1 ===
-
* pOmpC + TATA_INSA
 
-
** pOmpC ('''re-cultured, 10/2''')
 
** TATA_INSA ('''X.P. digestion, 10/2''')
** TATA_INSA ('''X.P. digestion, 10/2''')
-
* TATB_INSB + CinR+HSL+D-term
 
** TATB_INSB ('''E.S. digestion, 10/2''')
** TATB_INSB ('''E.S. digestion, 10/2''')
** CinR+HSL+D-term ('''E. digestion, 10/2''')
** CinR+HSL+D-term ('''E. digestion, 10/2''')
-
* (pOmpC + TATA_INSA) + (TATB_INSB + CinR+HSL+D-term)
 
-
** (pOmpC + TATA_INSA)
 
-
** (TATB_INSB + CinR+HSL+D-term)
 
=== System 2 ===
=== System 2 ===

Revision as of 13:43, 3 October 2007

GEL check for digestions

System 1

    • TATA_INSA (X.P. digestion, 10/2)
    • TATB_INSB (E.S. digestion, 10/2)
    • CinR+HSL+D-term (E. digestion, 10/2)

System 2

  • pCinRHSL + OmpRBS
    • pCinRHSL (re-cultured, 10/2)
    • OmpRBS (X. P. digestion, 10/2)
  • (pCinRHSL + OmpRBS) + TATD_IDE
    • (pCinRHSL + OmpRBS)
    • TATD_IDE
  • [(pCinRHSL + OmpRBS) + TATD_IDE] + D-term
    • [(pCinRHSL + OmpRBS) + TATD_IDE]
    • D-term