NYMU Taipei/Lab Notes/2007 10 5

From 2007.igem.org

(Difference between revisions)
(PCR length check)
(PCR length check)
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== PCR length check ==
== PCR length check ==
 +
* PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
* expected lengths of three ligations
* expected lengths of three ligations
** (I) pCinRHSL (vector) + OmpRBS (insert):  
** (I) pCinRHSL (vector) + OmpRBS (insert):  
** (II) pOmpC (vector) + TATA_INSA (insert)
** (II) pOmpC (vector) + TATA_INSA (insert)
** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)
** (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)

Revision as of 08:21, 5 October 2007

PCR length check

  • PCR product length without any insert = (3,189 - 3,049) + 176 = 316 by primers VF2 and VR
  • expected lengths of three ligations
    • (I) pCinRHSL (vector) + OmpRBS (insert):
    • (II) pOmpC (vector) + TATA_INSA (insert)
    • (III) TATB_INSB (insert) + CinR+HSL+D-term (vector)