NYMU Taipei/Lab Notes/2007 8 6&7

From 2007.igem.org

(Difference between revisions)
Line 9: Line 9:
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GGACATGGGTGTGTAGAAGA</font>
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GGACATGGGTGTGTAGAAGA</font>
* purchase of BCRC human insulin clone (ordered not paid)
* purchase of BCRC human insulin clone (ordered not paid)
 +
* antibiotics LB plate preparation
 +
** ampicillin
 +
** kanamycin

Revision as of 14:07, 6 August 2007

Lab schedule

  • mouse insulin gene cloning (delay to 8/7)
    • primer design for insulin II of Mus musculus
      • primer for A chain
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
      • primer for B chain
        • left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
        • right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
  • purchase of BCRC human insulin clone (ordered not paid)
  • antibiotics LB plate preparation
    • ampicillin
    • kanamycin
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