NYMU Taipei/Lab Notes/2007 8 6&7
From 2007.igem.org
(Difference between revisions)
Line 4: | Line 4: | ||
*** primer for A chain | *** primer for A chain | ||
**** translation (90-110): GIVDQCCTSICSLYQLENYCN | **** translation (90-110): GIVDQCCTSICSLYQLENYCN | ||
- | **** transcript (341-403): GGCATTGTAGATCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAAC | + | **** transcript (341-403): GGCATTGTAGATCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAAC, 63 bp |
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font> | **** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">ATTGTAGATCAGTGCTGCAC</font> | ||
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font> | **** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GTTGCAGTAGTTCTCCAGCT</font> | ||
*** primer for B chain | *** primer for B chain | ||
+ | **** translation (25-54): FVKQHLCGSHLVEALYLVCGERGFFYTPMS | ||
+ | **** transcript (146-235):TTTGTCAAGCAGCACCTTTGTGGTTCCCACCTGGTGGAGGCTCTCTACCTGGTGTGTGGGGAGCGTGGCTTCTTCTACACACCCATGTCC, 90 bp | ||
**** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">TTTGTCAAGCAGCACCTTTG</font> | **** left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATG<font color="red">TTTGTCAAGCAGCACCTTTG</font> | ||
**** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GGACATGGGTGTGTAGAAGA</font> | **** right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA<font color="red">GGACATGGGTGTGTAGAAGA</font> |
Revision as of 14:22, 6 August 2007
Lab schedule
- mouse insulin gene cloning (delay to 8/7)
- primer design for insulin II of Mus musculus, MN_008387.3 transcript
- primer for A chain
- translation (90-110): GIVDQCCTSICSLYQLENYCN
- transcript (341-403): GGCATTGTAGATCAGTGCTGCACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAAC, 63 bp
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATTGTAGATCAGTGCTGCAC
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT
- primer for B chain
- translation (25-54): FVKQHLCGSHLVEALYLVCGERGFFYTPMS
- transcript (146-235):TTTGTCAAGCAGCACCTTTGTGGTTCCCACCTGGTGGAGGCTCTCTACCTGGTGTGTGGGGAGCGTGGCTTCTTCTACACACCCATGTCC, 90 bp
- left: GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG
- right: GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA
- primer for A chain
- primer design for insulin II of Mus musculus, MN_008387.3 transcript
- purchase of BCRC human insulin clone (ordered not paid)
- antibiotics LB plate preparation
- ampicillin
- kanamycin