NYMU Taipei/Lab Notes/2007 8 9

From 2007.igem.org

(Difference between revisions)
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***[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription  
***[http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription  
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*Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
+
*Transformation to competent cell (Add <font color="red">2uL</font> vector to 50uL ECOS competent cells)
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
**Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)
**Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)

Revision as of 13:06, 9 August 2007

  • After first transformation
    • Pick single colony
      • [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] OmpR
      • [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] Constitutive Promoter, High Transcription
  • Transformation to competent cell (Add 2uL vector to 50uL ECOS competent cells)
    • Part:[http://partsregistry.org/Part:BBa_C0082 BBa_C0082], Receptor, tar-envZ at 7O, Plate 2
    • Part:[http://partsregistry.org/Part:BBa_I14032 BBa_I14032], Constitutive Promoter, High Transcription at 17K, Plate 2 (Transformation again)