NYMU Taipei/Lab Notes/2007 9 27

From 2007.igem.org

(Difference between revisions)
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* After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate
* After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate
* PCR check using VF2, VR primers
* PCR check using VF2, VR primers
 +
* PCR total 50 uL, 10 uL for GEL separation
[[Image:NYMU Taipei 20070927 CinR-HSL-D-term plate culture test.jpg]]
[[Image:NYMU Taipei 20070927 CinR-HSL-D-term plate culture test.jpg]]
* sample at each lane
* sample at each lane
** 1Kb ladder
** 1Kb ladder
-
** block #1
+
** block #1 (insert band appears)
-
** block #2
+
** block #2 (insert band appears, and one other band around 400 bp)
-
** block #3
+
** block #3 ()
** block #4
** block #4
** block #5
** block #5
** block #6
** block #6
-
* expect results
+
* expect results: one band at 1,623 bp is our insert
-
** one band at
+
** insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
 +
** insert = 790 + 730 + 103 = 1,623

Revision as of 09:10, 27 September 2007

  • After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate
  • PCR check using VF2, VR primers
  • PCR total 50 uL, 10 uL for GEL separation

NYMU Taipei 20070927 CinR-HSL-D-term plate culture test.jpg

  • sample at each lane
    • 1Kb ladder
    • block #1 (insert band appears)
    • block #2 (insert band appears, and one other band around 400 bp)
    • block #3 ()
    • block #4
    • block #5
    • block #6
  • expect results: one band at 1,623 bp is our insert
    • insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
    • insert = 790 + 730 + 103 = 1,623