NYMU Taipei/Lab Notes/2007 9 27

From 2007.igem.org

(Difference between revisions)
Line 6: Line 6:
** 1Kb ladder
** 1Kb ladder
** block #1 (insert band appears)
** block #1 (insert band appears)
-
** block #2 (insert band appears, and one other band around 400 bp)
+
** block #2 (insert band appears, and one extra band around 400 bp)
-
** block #3 ()
+
** block #3 (insert band appears)
-
** block #4
+
** block #4 (insert band appears)
-
** block #5
+
** block #5 (insert band does not appear, only band around 400 bp)
-
** block #6
+
** block #6 (insert band does not appear, only band around 400 bp)
* expect results: one band at 1,623 bp is our insert
* expect results: one band at 1,623 bp is our insert
** insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
** insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
** insert = 790 + 730 + 103 = 1,623
** insert = 790 + 730 + 103 = 1,623

Revision as of 09:11, 27 September 2007

  • After re-culture in 20-block plate,randomly pick 6 blocks from CinR+HSL+D-term plate
  • PCR check using VF2, VR primers
  • PCR total 50 uL, 10 uL for GEL separation

NYMU Taipei 20070927 CinR-HSL-D-term plate culture test.jpg

  • sample at each lane
    • 1Kb ladder
    • block #1 (insert band appears)
    • block #2 (insert band appears, and one extra band around 400 bp)
    • block #3 (insert band appears)
    • block #4 (insert band appears)
    • block #5 (insert band does not appear, only band around 400 bp)
    • block #6 (insert band does not appear, only band around 400 bp)
  • expect results: one band at 1,623 bp is our insert
    • insert = RBS+CinR (2,843 - 2,053) + RBS+HSL (2,783 - 2,053) + D-term (3,266 - 3,163)
    • insert = 790 + 730 + 103 = 1,623