NYMU Taipei/Lab Notes/2007 9 29

From 2007.igem.org

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PCR for TAT_B
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{| align=left | border=1 | style="text-align:center"
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!
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!
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!size
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!Primer
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!Template
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|-
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|3
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|TAT_B
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|137bp
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|TAT_F+TAT_B_R
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|E.coli K12 genome DNA
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Revision as of 07:45, 30 September 2007

PCR for TAT_A INS_A TAT_B INS_B TAT_D OmpRBS

size Primer Template
1 TAT_A 140bp TAT_F+TAT_A_R E.coli K12 genome DNA
2 INS_A 111bp INS_A_F+INS_A_R MGC clone (H_INS 1)
3 TAT_B 137bp TAT_F+TAT_B_R E.coli K12 genome DNA
4 INS_B 135bp INS_B_F+INS_B_R MGC clone (H_INS 1)
5 TAT_D 131bp TAT_F+TAT_D_R E.coli K12 genome DNA
6 OmpRBS 100bp OmpRBS_F+OmpRBS_R pOmpC
Template 1uL
10X buffer 5uL
2.5mM dNTP 2uL
Primer_F 1.5uL
Primer_R 1.5uL
Pfu polymerase 0.5uL
ddH2O 38.5uL
Total 50uL
Step Temp (°C) Time (mins) Cycle
Initial Denaturation 95 10
Denatuation
Annealing
Extension 		95
55
72 		1
1
1 		35
Final Extension 72 10
Soak 4



PCR for IDE

size Primer Template
IDE 3105bp IDE_F+IDE_R M_IDE
Template 1uL
10X buffer 5uL
2.5mM dNTP 2uL
Primer_F 1.5uL
Primer_R 1.5uL
Pfu polymerase 0.5uL
ddH2O 38.5uL
Total 50uL
Step Temp (°C) Time (mins) Cycle
Initial Denaturation 95 10
Denatuation
Annealing
Extension 		95
55
72 		1
1
4 		35
Final Extension 72 10
Soak 4

PCR for TAT_B

size Primer Template
3 TAT_B 137bp TAT_F+TAT_B_R E.coli K12 genome DNA

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