NYMU Taipei/Lab Notes/2007 9 29
From 2007.igem.org
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+ | {| align=center | border=1 | style="text-align:center" | ||
+ | |||
+ | |Template | ||
+ | |1uL | ||
+ | |- | ||
+ | |10X buffer | ||
+ | |5uL | ||
+ | |- | ||
+ | |2.5mM dNTP | ||
+ | |2uL | ||
+ | |- | ||
+ | |Primer_F | ||
+ | |1.5uL | ||
+ | |- | ||
+ | |Primer_R | ||
+ | |1.5uL | ||
+ | |- | ||
+ | |pfu polymerase | ||
+ | |0.5uL | ||
+ | |- | ||
+ | |ddH2O | ||
+ | |38.5uL | ||
+ | |- | ||
+ | |Total | ||
+ | |50uL | ||
+ | |- |
Revision as of 12:40, 29 September 2007
PCR
size | Primer | Template | ||
---|---|---|---|---|
1 | TAT_A | 140bp | TAT_F+TAT_A_R | E.coli K12 genome DNA |
2 | INS_A | 111bp | INS_A_F+INS_A_R | MGC clone (H_INS 1) |
3 | TAT_B | 137bp | TAT_F+TAT_B_R | E.coli K12 genome DNA |
4 | INS_B | 135bp | INS_B_F+INS_B_R | MGC clone (H_INS 1) |
5 | TAT_D | 131bp | TAT_F+TAT_D_R | E.coli K12 genome DNA |
6 | OmpRBS | 100bp | OmpRBS_F+OmpRBS_R | pOmpC |
Template | 1uL |
10X buffer | 5uL |
2.5mM dNTP | 2uL |
Primer_F | 1.5uL |
Primer_R | 1.5uL |
pfu polymerase | 0.5uL |
ddH2O | 38.5uL |
Total | 50uL |