NYMU Taipei/Lab Notes/2007 9 29
From 2007.igem.org
(Difference between revisions)
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|Total | |Total | ||
|50uL | |50uL | ||
- | |- | + | |-} |
Revision as of 12:44, 29 September 2007
PCR
size | Primer | Template | ||
---|---|---|---|---|
1 | TAT_A | 140bp | TAT_F+TAT_A_R | E.coli K12 genome DNA |
2 | INS_A | 111bp | INS_A_F+INS_A_R | MGC clone (H_INS 1) |
3 | TAT_B | 137bp | TAT_F+TAT_B_R | E.coli K12 genome DNA |
4 | INS_B | 135bp | INS_B_F+INS_B_R | MGC clone (H_INS 1) |
5 | TAT_D | 131bp | TAT_F+TAT_D_R | E.coli K12 genome DNA |
6 | OmpRBS | 100bp | OmpRBS_F+OmpRBS_R | pOmpC |
Template | 1uL |
10X buffer | 5uL |
2.5mM dNTP | 2uL |
Primer_F | 1.5uL |
Primer_R | 1.5uL |
pfu polymerase | 0.5uL |
ddH2O | 38.5uL |
Total | 50uL |