NYMU Taipei/Materials

From 2007.igem.org

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                 <li><font size="2">[http://strain.bcrc.firdi.org.tw/BSAS/controller?event=SEARCH&amp;bcrc_no=g1005043C07&amp;type_id=5&amp;keyword=insulin </font><strong>g1005043C07: </strong>MGC Human Verified Full-length cDNA Collection (IRAU), IMAGE id=3950204<font size="2">] (from [http://www.bcrc.firdi.org.tw/wwwbcrc/main.jsp BCRC], 4,000 NTD)</font></li>
                 <li><font size="2">[http://strain.bcrc.firdi.org.tw/BSAS/controller?event=SEARCH&amp;bcrc_no=g1005043C07&amp;type_id=5&amp;keyword=insulin </font><strong>g1005043C07: </strong>MGC Human Verified Full-length cDNA Collection (IRAU), IMAGE id=3950204<font size="2">] (from [http://www.bcrc.firdi.org.tw/wwwbcrc/main.jsp BCRC], 4,000 NTD)</font></li>
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                 <li><font size="2">[[Construct details]]</font></li>
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                 <li><font size="2">[https://2007.igem.org/NYMU_Taipei/Costruct Construct details]</font></li>
                 <li><font size="2">[http://www.dls.ym.edu.tw/t11.htm 生科系蔡亭芬老師</font><font size="2">] 可提供老鼠 (BL_4) 胰臟組織做 RT-PCR 取 insulin clone (連絡日期 8/3, PM 4:28)</font>
                 <li><font size="2">[http://www.dls.ym.edu.tw/t11.htm 生科系蔡亭芬老師</font><font size="2">] 可提供老鼠 (BL_4) 胰臟組織做 RT-PCR 取 insulin clone (連絡日期 8/3, PM 4:28)</font>
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Revision as of 12:51, 19 October 2007

Contents

What we need in our project


Beginning of Story

  • De-Ming Lin had concluded what a insulin-secretory cell should be from interature review
    • Cells engineered for insulin expression should contain
      • Proteases for insuline or proinsulin or express two chains (A, B) separately (activate insulin)
      • glucose-sensing system (sense glucose)
      • secretory granules that can release insulin promptly by exocytosis in response to extracellular glucose levels

Action plan

 

       RNA Switch Biosensor 

  1. Influenza virus like particle (VLP)-get from professor Ho, Mei-Shang (Academia Sinica, IBMS )
  2. iNOS promoter and iNOS gene construct-get from professor Lin, Wan-Wan (NTU)
  3. pTREX -GFP construct-get from professor Stephen Elledge (Harvard University)
  4. Cytochrome c- from VYMGC
  5. Antiswitch, TetR, pTet-get from iGEM

 


Make for practical use

  • control the population of E.coli-based insulin secretory cell
  • prevent the immune response

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