NYMU Taipei/Part List
From 2007.igem.org
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|OmpR binding sites | |OmpR binding sites | ||
| - | | | + | |65 |
| | | | ||
|? | |? | ||
| Line 117: | Line 117: | ||
|This part accepts POPS to generate EYFP under the medium strength RBS B0032 | |This part accepts POPS to generate EYFP under the medium strength RBS B0032 | ||
|742 | |742 | ||
| + | |pSB1A2 | ||
| + | |OK | ||
| + | |- | ||
| + | |[http://partsregistry.org/Part:BBa_E0840 BBa_E0840] | ||
| + | |B0030(RBS).E0040(GFP).B0015(D-term) | ||
| + | |16E, plate 1 | ||
| + | |gfp tri-part; strong rbs | ||
| + | |878 | ||
|pSB1A2 | |pSB1A2 | ||
|OK | |OK | ||
| Line 158: | Line 166: | ||
* A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin] | * A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin] | ||
** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin | ** refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin | ||
| - | ** stock (100 mg/mL = 100 ug/uL) | + | ** stock (100 mg/mL = 100 ug/uL) ampicillin |
** liquid culture (50 ug/mL = 250 ug/5mL) | ** liquid culture (50 ug/mL = 250 ug/5mL) | ||
| - | ** 5mL liquid culture = 5 c.c. LB + 2.5uL stock | + | ** 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin |
* K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin] | * K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin] | ||
* C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol] | * C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol] | ||
Latest revision as of 09:08, 24 January 2008
Project Description /
Lab Notes /
Part List /
Primer List
Part List
| Series | alias | Location | Description | part length | vector (antibotics) | Check |
|---|---|---|---|---|---|---|
| [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] | pOmpC | 1 | Positively regulated, OmpR-controlled promoter | 108 | pSB1A2 | ? |
| [http://partsregistry.org/Part:BBa_C0082 BBa_C0082] | tar-envZ | 1 | Receptor, tar-envZ | 1491 | pSB2K3 | |
| [http://partsregistry.org/Part:BBa_B0034 BBa_B0034] | RBS | 1,2,3 | The Elowitz RBS (Ribosome binding site) is the definition of efficiency 1.0 | 12 | pSB1A2 | ? |
| AU43-C7 | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=13528923 INS] | human insulin | 495 | C | ||
| INS_A | 1 | alpha chain of human insulin | 63 | |||
| INS_B | 1 | beta chain of human insulin | 90 | |||
| [http://partsregistry.org/Part:BBa_B0014 BBa_B0014] | D-term | 1,2,3 | Double transcription terminator | 95 | pSB1AK3 | OK |
| [http://partsregistry.org/Part:BBa_I14032 BBa_I14032] | pLacIQ | 2 | Constitutive Promoter, High Transcription | 37 | pSB2K3 | |
| OmpRBS | 2 | OmpR binding sites | 65 | ? | ||
| AV88-C6 | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=27371195 IDE] | 2 | insulin degradation enzyme of mouse | 3445 | A | |
| AU61-F6 | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=33879043 IGFBP3] | 2 | Insulin-like growth factor binding protein 3 of human | 2488 | C | |
| AU50-C7 | [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=38114635 IGFBP7] | 2 | Insulin-like growth factor binding protein 7 of human | 1155 | C | |
| [http://partsregistry.org/Part:BBa_I15016 BBa_I15016] | B0032.ECFP | 16D, plate 1 | This part accepts POPS to produce ECFP under the medium RBS B0032 | 742 | pSB1A2 | OK |
| [http://partsregistry.org/Part:BBa_I15017 BBa_I15017] | B0032.EYFP | 16F, plate 1 | This part accepts POPS to generate EYFP under the medium strength RBS B0032 | 742 | pSB1A2 | OK |
| [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] | B0030(RBS).E0040(GFP).B0015(D-term) | 16E, plate 1 | gfp tri-part; strong rbs | 878 | pSB1A2 | OK |
| [http://partsregistry.org/Part:BBa_R0077 BBa_R0077] | pCinRHSL, RBS+ | 17L, Plate 1 | CinR-HSL complex binds to this promoter and activates transcription. This part contains RBS. | 231 | pSB1A2 | OK |
| [http://partsregistry.org/Part:BBa_R0078 BBa_R0078] | pCinRHSL | 15D, Plate 1 | CinR-HSL complex binds to this promoter and activates transcription. This part DOES NOT contain RBS. | 225 | pSB1A2 | ? |
| [http://partsregistry.org/Part:BBa_C0077 BBa_C0077] | CinR | 7G, Plate 2 | cinR activator | 762 | pSB2K3 | OK |
| [http://partsregistry.org/Part:BBa_C0076 BBa_C0076] | HSL | 7E, Plate 2 | autoinducer | 702 | pSB2K3 | OK |
antibiotics keys
- A: [http://en.wikipedia.org/wiki/Ampicillin Ampicillin]
- refernece at OpenWetWare: http://openwetware.org/wiki/Ampicillin
- stock (100 mg/mL = 100 ug/uL) ampicillin
- liquid culture (50 ug/mL = 250 ug/5mL)
- 5mL liquid culture = 5 c.c. LB + 2.5uL stock ampicillin
- K: [http://en.wikipedia.org/wiki/Kanamycin Kanamycin]
- C: [http://en.wikipedia.org/wiki/Chloramphenicol Chloramphenicol]
- 35 mg/mL
- 20 mg/mL in Ethanol
- we prepared 100mg in 5 mL and stored in -20 C
