NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 9: Line 9:
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|-
|M_INS_A 5'
|M_INS_A 5'
-
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
+
|<font color="green">GTTTCTTCGAATTCGCGGCCGCTTCTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
|51 bp
|51 bp
-
|
+
|56.85
|EcoRI, XbaI
|EcoRI, XbaI
|63 bp
|63 bp
Line 20: Line 20:
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
|55 bp
|55 bp
-
|
+
|54.74
|SpeI, PstI
|SpeI, PstI
|63 bp
|63 bp
Line 68: Line 68:
|
|
|Forward sequencing primer.
|Forward sequencing primer.
 +
|-
 +
|-
 +
|[http://partsregistry.org/Part:BBa_G00101 VR]
 +
|attaccgcctttgagtgagc
 +
|20 bp
 +
|59.34
 +
|
 +
|
 +
|Reverse sequencing primer.
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|-

Revision as of 09:32, 17 August 2007

Name Sequence Length Tm Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG 51 bp 56.85 EcoRI, XbaI 63 bp Potential dimer formation!
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 55 bp 54.74 SpeI, PstI 63 bp Potential dimer formation!
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 90 bp
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 55 bp 55.86 SpeI, PstI 90 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1396 bp
RcsC 3'-2 AAGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1396 bp Two extra adenosine was added to the 5' side of the NdeI cutting site.
[http://partsregistry.org/Part:BBa_G00100 VF2] tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
[http://partsregistry.org/Part:BBa_G00101 VR] attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
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