NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 13: Line 13:
|56.85
|56.85
|EcoRI, XbaI
|EcoRI, XbaI
-
|63 bp
+
|126 bp
|Potential dimer formation!
|Potential dimer formation!
|-
|-
Line 22: Line 22:
|54.74
|54.74
|SpeI, PstI
|SpeI, PstI
-
|63 bp
+
|126 bp
|Potential dimer formation!
|Potential dimer formation!
|-
|-

Revision as of 09:13, 21 August 2007

Name Sequence Length Tm (C) Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG 51 bp 56.85 EcoRI, XbaI 126 bp Potential dimer formation!
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 55 bp 54.74 SpeI, PstI 126 bp Potential dimer formation!
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT 52 bp 60.85 EcoRI, XbaI 156 bp Potential dimer formation!
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 55 bp 55.86 SpeI, PstI 156 bp Potential dimer formation!
H_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG 51 bp 62.45 EcoRI, XbaI 129 bp Potential dimer formation!
H_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 56 bp 57.77 SpeI, PstI 129 bp Potential dimer formation!
H_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG 51 bp 57.98 EcoRI, XbaI 156 bp Potential dimer formation!
H_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 55 bp 55.10 SpeI, PstI 156 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1404 bp
RcsC 3'-2 GGGGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1404 bp Three extra guanosine was added to the 5' side of the NdeI cutting site.
VF2 tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
VR attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
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