NYMU Taipei/Primer List
From 2007.igem.org
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[[NYMU_Taipei/ProjectDescription| Project Description]] / | [[NYMU_Taipei/ProjectDescription| Project Description]] / | ||
| + | [[NYMU_Taipei/Lab_Notes| Lab Notes]] / | ||
[[NYMU_Taipei/Part_List| Part List]] / | [[NYMU_Taipei/Part_List| Part List]] / | ||
[[NYMU_Taipei/Primer_List| Primer List]] | [[NYMU_Taipei/Primer_List| Primer List]] | ||
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Revision as of 06:51, 24 August 2007
Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | Restriction site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTAGATCAGTGCTG | 51 bp | 56.85 | EcoRI, XbaI | 119 bp | Potential dimer formation! |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTGT | 52 bp | 60.85 | EcoRI, XbaI | 149 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGGCATTGTGGAACAATGCTG | 51 bp | 62.45 | EcoRI, XbaI | 122 bp | Potential dimer formation! |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTGAACCAACACCTGTG | 51 bp | 57.98 | EcoRI, XbaI | 149 bp | Potential dimer formation! |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1404 bp | |
| RcsC 3'-2 | GGGGTATACCAACGACTCTTCCATCTTCAC | 29 bp | 59.63 | NdeI | 1404 bp | Three extra guanosine was added to the 5' side of the NdeI cutting site. |
| VF2 | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| VR | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
