NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 15: Line 15:
!Description
!Description
|-
|-
-
|M_INS_A 5'
+
|M_INS_A F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font>
|54 bp
|54 bp
Line 24: Line 24:
|-
|-
|-
|-
-
|M_INS_A 3'
+
|M_INS_A R
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCT</font>
|48 bp
|48 bp
|54.74
|54.74
|SpeI, PstI
|SpeI, PstI
|119 bp
|119 bp
-
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
+
|Potential dimer formation!
|-
|-
-
|M_INS_B 5'
+
|M_INS_B F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font>
|55 bp
|55 bp
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|-
|-
|-
|-
-
|M_INS_B 3'
+
|M_INS_B R
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGACATGGGTGTGTAGAAGA</font>
|48 bp
|48 bp
|55.86
|55.86
|SpeI, PstI
|SpeI, PstI
|149 bp
|149 bp
-
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
+
|Potential dimer formation!
|-
|-
|-
|-
-
|H_INS_A 5'
+
|H_INS_A F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|54 bp
|54 bp
Line 59: Line 59:
|-
|-
|-
|-
-
|H_INS_A 3'
+
|H_INS_A R'
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GTTGCAGTAGTTCTCCAGCTG</font>
|49 bp
|49 bp
|57.77
|57.77
|SpeI, PstI
|SpeI, PstI
|122 bp
|122 bp
-
|Potential dimer formation! Stop codons are removed to enable fusion with export signal.
+
|Potential dimer formation!
|-
|-
-
|H_INS_B 5'
+
|H_INS_B F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font>
|54 bp
|54 bp
Line 76: Line 76:
|-
|-
|-
|-
-
|H_INS_B 3'
+
|H_INS_B R
-
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGT</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
+
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GGTCTTGGGTGTGTAGAAGA</font>
|48 bp
|48 bp
|55.10
|55.10
|SpeI, PstI
|SpeI, PstI
|149 bp
|149 bp
-
|Stop codons are removed to enable fusion with export signal.
+
|
|-
|-
|-
|-
-
|RcsC 5'
+
|RcsC F
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAAATACCTTGCTTCTTTTCG</font>
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAAATACCTTGCTTCTTTTCG</font>
|54 bp
|54 bp
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|-
|-
|-
|-
-
|RcsC 3'-2
+
|RcsC R
|<font color="green">GTTTCTTCCATATG</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
|<font color="green">GTTTCTTCCATATG</font><font color="red">CAACGACTCTTCCATCTTCAC</font>
|34 bp
|34 bp
Line 119: Line 119:
|
|
|Reverse sequencing primer.
|Reverse sequencing primer.
 +
|-
 +
|-M_IDE F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
 +
|54 bp
 +
|
 +
|XbaI
 +
| bp
 +
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|-
 +
|M_IDE R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">GAGTTTTGCAGCCATGAAGT</font>
 +
|48 bp
 +
|54.74
 +
|SpeI, PstI
 +
| bp
 +
|Potential dimer formation!
 +
|-
 +
|-H_IGFBP3 F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CAGCGGGCGCGACCCACGCT</font>
 +
|54 bp
 +
|
 +
|XbaI
 +
| bp
 +
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|-
 +
|H_IGFBP3 R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">CTTGCTCTGCATGCTGTAGC</font>
 +
|48 bp
 +
|54.74
 +
|SpeI, PstI
 +
| bp
 +
|Potential dimer formation!
 +
|-
 +
|-H_IGFBP7 F
 +
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GAGCGGCCGTCGCTGCGCGC</font>
 +
|54 bp
 +
|
 +
|XbaI
 +
| bp
 +
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|-
 +
|H_IGFBP7 R
 +
|<font color="green">GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTA</font><font color="red">TAGCTCGGCACCTTCACCTT</font>
 +
|48 bp
 +
|54.74
 +
|SpeI, PstI
 +
| bp
 +
|Potential dimer formation!
|-
|-
|}
|}
* [http://www.mdbio.com.tw/pfu.htm pfu primer]
* [http://www.mdbio.com.tw/pfu.htm pfu primer]

Revision as of 09:15, 31 August 2007

Project Description / Lab Notes / Part List / Primer List


Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
VF2 tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
VR attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCGGAACGGGCTCGTGTGGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!