NYMU Taipei/Primer List

From 2007.igem.org

(Difference between revisions)
Line 59: Line 59:
|-
|-
|H_INS_A F
|H_INS_A F
-
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
+
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font>
|54 bp
|54 bp
|62.45
|62.45
|XbaI
|XbaI
|122 bp
|122 bp
-
|Potential dimer formation! XbaI+RBS, ATG removed.
+
|Potential dimer formation! XbaI+RBS
|-
|-
|H_INS_A TAT F
|H_INS_A TAT F
Line 74: Line 74:
|
|
|-
|-
-
|TAT signal H_INS_A R
+
|TAT H_INS_A R
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
|<font color="green">CAGCATTGTTCCACAATGCC</font><font color="red">CGCTTGCGCCGC</font>
|32 bp
|32 bp
Line 97: Line 97:
|149 bp
|149 bp
|Potential dimer formation! XbaI+RBS
|Potential dimer formation! XbaI+RBS
 +
|-
 +
|H_INS_B TAT F
 +
|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">TTTGTGAACCAACACCTGTG</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
 +
|-
 +
|TAT H_INS_B R
 +
|<font color="green">CACAGGTGTTGGTTCACAAA</font><font color="red">CGCTTGCGCCGC</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
 +
|-
|-
|-
|-
|-
Line 143: Line 160:
|Reverse sequencing primer.
|Reverse sequencing primer.
|-
|-
-
|M_IDE F
+
|M_IDE TAT F
-
|<font color="green">GTTTCTTCTCTAGAaaagaggagaaaACTAGATG</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
+
|<font color="green">CGCTTGCGCCGCAGTCGCAC</font><font color="red">CGGAACGGGCTCGTGTGGCT</font>
-
|54 bp
+
|40 bp
 +
|
|
|
-
|XbaI
 
| bp
| bp
-
|Potential dimer formation! XbaI+RBS
+
|Potential dimer formation!
 +
|-
 +
|TAT M_IDE R
 +
|<font color="green">AGCCACACGAGCCCGTTCCG</font><font color="red">CGCTTGCGCCGC</font>
 +
|bp
 +
|
 +
|
 +
|bp
 +
|
 +
|-
|-
|-
|-
|-

Revision as of 10:28, 13 September 2007

Project Description / Lab Notes / Part List / Primer List

Name Sequence Length Tm (C) RE site Target length Description
M_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTAGATCAGTGCTG 54 bp 56.85 XbaI 119 bp Potential dimer formation! XbaI+RBS
M_INS_A R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 48 bp 54.74 SpeI, PstI 119 bp Potential dimer formation!
M_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTCAAGCAGCACCTTTGT 55 bp 60.85 XbaI 149 bp Potential dimer formation!
M_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 48 bp 55.86 SpeI, PstI 149 bp Potential dimer formation!
TAT F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGAACAATAACGATCTCTTTCAGG 56 bp 59.92 XbaI bp XbaI+RBS
H_INS_A F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGGCATTGTGGAACAATGCTG 54 bp 62.45 XbaI 122 bp Potential dimer formation! XbaI+RBS
H_INS_A TAT F CGCTTGCGCCGCAGTCGCACGGCATTGTGGAACAATGCTG bp bp
TAT H_INS_A R CAGCATTGTTCCACAATGCCCGCTTGCGCCGC 32 bp 59.26 bp Potential dimer formation!
H_INS_A R' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCTG 49 bp 57.77 SpeI, PstI 122 bp Potential dimer formation!
H_INS_B F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGTTTGTGAACCAACACCTGTG 54 bp 57.98 XbaI 149 bp Potential dimer formation! XbaI+RBS
H_INS_B TAT F CGCTTGCGCCGCAGTCGCACTTTGTGAACCAACACCTGTG bp bp
TAT H_INS_B R CACAGGTGTTGGTTCACAAACGCTTGCGCCGC bp bp
H_INS_B R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGTCTTGGGTGTGTAGAAGA 48 bp 55.10 SpeI, PstI 149 bp
RcsC F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAAATACCTTGCTTCTTTTCG 54 bp 55.92 XbaI 1404 bp XbaI+RBS
RcsC R GTTTCTTCCATATGCAACGACTCTTCCATCTTCAC 34 bp 59.63 NdeI 1404 bp BioBrick standard spacer was added to the 5' side of the NdeI cutting site.
VF2 tgccacctgacgtctaagaa 20 bp 59.44 Forward sequencing primer.
VR attaccgcctttgagtgagc 20 bp 59.34 Reverse sequencing primer.
M_IDE TAT F CGCTTGCGCCGCAGTCGCACCGGAACGGGCTCGTGTGGCT 40 bp bp Potential dimer formation!
TAT M_IDE R AGCCACACGAGCCCGTTCCGCGCTTGCGCCGC bp bp
M_IDE R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAGTTTTGCAGCCATGAAGT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP3 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGCAGCGGGCGCGACCCACGCT 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP3 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTGCTCTGCATGCTGTAGC 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
H_IGFBP7 F GTTTCTTCTCTAGAaaagaggagaaaACTAGATGGAGCGGCCGTCGCTGCGCGC 54 bp XbaI bp Potential dimer formation! XbaI+RBS
H_IGFBP7 R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATAGCTCGGCACCTTCACCTT 48 bp 54.74 SpeI, PstI bp Potential dimer formation!
Retrieved from "http://2007.igem.org/wiki/index.php/NYMU_Taipei/Primer_List"