NYMU Taipei/Primer List
From 2007.igem.org
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!Length | !Length | ||
!Tm (C) | !Tm (C) | ||
| - | ! | + | !RE site |
!Target length | !Target length | ||
!Description | !Description | ||
|- | |- | ||
|M_INS_A 5' | |M_INS_A 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">GGCATTGTAGATCAGTGCTG</font> |
| - | | | + | |54 bp |
|56.85 | |56.85 | ||
| - | | | + | |XbaI |
|119 bp | |119 bp | ||
| - | |Potential dimer formation! | + | |Potential dimer formation! XbaI+RBS |
|- | |- | ||
|- | |- | ||
| Line 33: | Line 33: | ||
|- | |- | ||
|M_INS_B 5' | |M_INS_B 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTCAAGCAGCACCTTTGT</font> |
|52 bp | |52 bp | ||
|60.85 | |60.85 | ||
| Line 51: | Line 51: | ||
|- | |- | ||
|H_INS_A 5' | |H_INS_A 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">GGCATTGTGGAACAATGCTG</font> |
|51 bp | |51 bp | ||
|62.45 | |62.45 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
|122 bp | |122 bp | ||
| - | |Potential dimer formation! | + | |Potential dimer formation! XbaI+RBS |
|- | |- | ||
|- | |- | ||
| Line 68: | Line 68: | ||
|- | |- | ||
|H_INS_B 5' | |H_INS_B 5' | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTC TCTAGA aaagaggagaaa ACTAGATG</font><font color="red">TTTGTGAACCAACACCTGTG</font> |
|51 bp | |51 bp | ||
|57.98 | |57.98 | ||
|EcoRI, XbaI | |EcoRI, XbaI | ||
|149 bp | |149 bp | ||
| - | |Potential dimer formation! | + | |Potential dimer formation! XbaI+RBS |
|- | |- | ||
|- | |- | ||
| Line 95: | Line 95: | ||
|- | |- | ||
|RcsC 3'-2 | |RcsC 3'-2 | ||
| - | |<font color="green"> | + | |<font color="green">GTTTCTTCGTATAC</font><font color="red">CAACGACTCTTCCATCTTCAC</font> |
| - | | | + | |34 bp |
|59.63 | |59.63 | ||
|NdeI | |NdeI | ||
|1404 bp | |1404 bp | ||
| - | | | + | |BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
|- | |- | ||
|- | |- | ||
Revision as of 15:10, 24 August 2007
Project Description /
Lab Notes /
Part List /
Primer List
| Name | Sequence | Length | Tm (C) | RE site | Target length | Description |
|---|---|---|---|---|---|---|
| M_INS_A 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTAGATCAGTGCTG | 54 bp | 56.85 | XbaI | 119 bp | Potential dimer formation! XbaI+RBS |
| M_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCT | 48 bp | 54.74 | SpeI, PstI | 119 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| M_INS_B 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTCAAGCAGCACCTTTGT | 52 bp | 60.85 | EcoRI, XbaI | 149 bp | Potential dimer formation! |
| M_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGACATGGGTGTGTAGAAGA | 48 bp | 55.86 | SpeI, PstI | 149 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_A 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGGGCATTGTGGAACAATGCTG | 51 bp | 62.45 | EcoRI, XbaI | 122 bp | Potential dimer formation! XbaI+RBS |
| H_INS_A 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGTTGCAGTAGTTCTCCAGCTG | 49 bp | 57.77 | SpeI, PstI | 122 bp | Potential dimer formation! Stop codons are removed to enable fusion with export signal. |
| H_INS_B 5' | GTTTCTTC TCTAGA aaagaggagaaa ACTAGATGTTTGTGAACCAACACCTGTG | 51 bp | 57.98 | EcoRI, XbaI | 149 bp | Potential dimer formation! XbaI+RBS |
| H_INS_B 3' | GTTTCTTCCTGCAGCGGCCGCTACTAGTGGTCTTGGGTGTGTAGAAGA | 48 bp | 55.10 | SpeI, PstI | 149 bp | Stop codons are removed to enable fusion with export signal. |
| RcsC 5' | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG | 51 bp | 55.92 | EcoRI, XbaI | 1404 bp | |
| RcsC 3'-2 | GTTTCTTCGTATACCAACGACTCTTCCATCTTCAC | 34 bp | 59.63 | NdeI | 1404 bp | BioBrick standard spacer was added to the 5' side of the NdeI cutting site. |
| [http://partsregistry.org/Part:BBa_G00100 VF2] | tgccacctgacgtctaagaa | 20 bp | 59.44 | Forward sequencing primer. | ||
| [http://partsregistry.org/Part:BBa_G00101 VR] | attaccgcctttgagtgagc | 20 bp | 59.34 | Reverse sequencing primer. |
- [http://www.mdbio.com.tw/pfu.htm pfu primer]
