NYMU Taipei/Primer List

From 2007.igem.org

Revision as of 09:07, 17 August 2007 by Lihsiangyen (Talk | contribs)
Name Sequence Length Tm Restriction site Target length Description
M_INS_A 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGTTGCAGTAGTTCTCCAGCT 51 bp EcoRI, XbaI 63 bp Potential dimer formation! Need to design a new one.
M_INS_A 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGTTGCAGTAGTTCTCCAGCT 55 bp SpeI, PstI 63 bp Potential dimer formation! Need to design a new one.
M_INS_B 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTTTGTCAAGCAGCACCTTTG 51 bp 60.03 EcoRI, XbaI 63 bp Potential dimer formation! Need to design a new one.
M_INS_B 3' GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGACATGGGTGTGTAGAAGA 55 bp 55.86 SpeI, PstI 90 bp
RcsC 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAAATACCTTGCTTCTTTTCG 51 bp 55.92 EcoRI, XbaI 1396 bp
RcsC 3'-2 AAGTATACCAACGACTCTTCCATCTTCAC 29 bp 59.63 NdeI 1396 bp Two extra Adenosine was added to the 5' side of the NdeI cutting site.