PHO80cds
From 2007.igem.org
| Line 2: | Line 2: | ||
CODINGSEQUENCE | CODINGSEQUENCE | ||
| - | + | ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATG | |
| + | AGGATCAAGGGATACCAAAAGTAATTCTGCCCGCTGATTT | ||
| + | TAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCA | ||
| + | CGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAG | ||
| + | CAACAAAGAAATCTGATGACCAAATTACTTTAACACGATA | ||
| + | CCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTAT | ||
| + | TTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTG | ||
| + | TGCTTATGACATCACTCTATTATATCGATTTATTGCAAAC | ||
| + | TGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCAT | ||
| + | AGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCT | ||
| + | TATGTGATTCGTTCTCAACAAACGCCCATTATGCAAAAGT | ||
| + | TGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAAC | ||
| + | GATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGG | ||
| + | ATCATAACATTACGTTATGTAGTATAGAGCAAAAACAGAA | ||
| + | AAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGAT | ||
| + | TTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGAT | ||
| + | ATAATGTTCTAGATAAATACTATCGAAGAATAGTTCAGCT | ||
| + | GGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAG | ||
| + | GTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTG | ||
| + | AAAGTGGCTCCCAAACTACTCAACTAAAGGGGTCGTCATC | ||
| + | ACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCA | ||
| + | AAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATT | ||
| + | AA | ||
XbaI restriction sequence TCTAGA | XbaI restriction sequence TCTAGA | ||
cuts in sites | cuts in sites | ||
| - | + | 91 | |
| - | + | 648 | |
| Line 29: | Line 51: | ||
Frame 5’-3’ | Frame 5’-3’ | ||
| - | |||
| - | |||
VirtualSequence: | VirtualSequence: | ||
| - | |||
MESTSGERSENIHEDQGIPKVILPADFNKC[[SR]]TDLVVLISRMLVSLIAINENSATKKSDD | MESTSGERSENIHEDQGIPKVILPADFNKC[[SR]]TDLVVLISRMLVSLIAINENSATKKSDD | ||
QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH | QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH | ||
Revision as of 11:19, 17 October 2007
GenePho CODINGSEQUENCE
ATGGAAAGCACATCAGGAGAACGTTCCGAAAATATACATG AGGATCAAGGGATACCAAAAGTAATTCTGCCCGCTGATTT TAATAAATGCTCTAGAACTGACCTAGTGGTGCTCATATCA CGAATGTTAGTATCGCTGATAGCAATCAATGAAAATTCAG CAACAAAGAAATCTGATGACCAAATTACTTTAACACGATA CCATTCTAAGATTCCTCCAAACATATCAATCTTCAACTAT TTCATACGACTGACAAAGTTTTCCTCTTTAGAACATTGTG TGCTTATGACATCACTCTATTATATCGATTTATTGCAAAC TGTGTATCCTGATTTTACGCTTAATTCGTTGACTGCCCAT AGGTTTTTATTAACAGCCACCACAGTCGCAACAAAAGGCT TATGTGATTCGTTCTCAACAAACGCCCATTATGCAAAAGT TGGAGGAGTACGATGTCACGAATTGAATATACTGGAGAAC GATTTTTTAAAGAGAGTAAACTACAGAATCATTCCGCGGG ATCATAACATTACGTTATGTAGTATAGAGCAAAAACAGAA AAAGTTTGTCATAGATAAAAACGCATTAGGGTCTCTCGAT TTGGATTCTTATTCTTACGTTAATCGTCCAAAAAGTGGAT ATAATGTTCTAGATAAATACTATCGAAGAATAGTTCAGCT GGTGGGTTCCTTTAACGCTTCACCTGATAAGAGTAGAAAG GTTGATTATGTTCTCCCGCCAAATATTGATATAGTGAGTG AAAGTGGCTCCCAAACTACTCAACTAAAGGGGTCGTCATC ACCCAATTCTCACTCTTCACAAAAGCGATATTCTGAGGCA AAGGACGCACATATCTATAACAAGCGATCAAAGCCAGATT AA
XbaI restriction sequence TCTAGA cuts in sites 91 648
Pho cds 882bp e 293aa
1)91 bp position 31-32aa SR S=TCTTCCTCATCG
R=AGAAGG
2)648 bp position 216-217-218aa VLD V=TTGTCGTAGTG
L=TTATTGCTTCTACTCCTG
D=GATGAC
Frame 5’-3’ VirtualSequence: MESTSGERSENIHEDQGIPKVILPADFNKCSRTDLVVLISRMLVSLIAINENSATKKSDD QITLTRYHSKIPPNISIFNYFIRLTKFSSLEHCVLMTSLYYIDLLQTVYPDFTLNSLTAH RFLLTATTVATKGLCDSFSTNAHYAKVGGVRCHELNILENDFLKRVNYRIIPRDHNITLC SIEQKQKKFVIDKNALGSLDLDSYSYVNRPKSGYNVLDKYYRRIVQLVGSFNASPDKSRK VDYVLPPNIDIVSESGSQTTQLKGSSSPNSHSSQKRYSEAKDAHIYNKRSKPD
PHO80 Primer sequences:
t93c 5'-cgctgattttaataaatgctccagaactgacctagtggtgc-3'
t93c antisense 5'-gcaccactaggtcagttctggagcatttattaaaatcagcg-3'
t648c 5'-atcgtccaaaaagtggatataatgtcctagataaatactatcgaagaatag-3'
t648c antisense 5'-ctattcttcgatagtatttatctaggacattatatccactttttggacgat-3'
