Paris/DISCUSSION
From 2007.igem.org
Nicolas C. (Talk | contribs) |
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| - | *Test of DapA deletion transduction, Thursday, July 5 | + | *'''Test of DapA deletion transduction, Thursday, July 5 |
| - | + | ''' | |
How did you test for the success of this transduction? Simply with a normal culture without supplemented lysine in the medium, and seeing that the bacteria grows neverthless? Or is it that in the original DapA- strain tha DapA gene was replaced by an antibiotic resistance gene, that should then be present in the transfected bacteria? | How did you test for the success of this transduction? Simply with a normal culture without supplemented lysine in the medium, and seeing that the bacteria grows neverthless? Or is it that in the original DapA- strain tha DapA gene was replaced by an antibiotic resistance gene, that should then be present in the transfected bacteria? | ||
| - | *For the modellers | + | *'''For the modellers''' |
Have a look at the Cambridge team wiki. They have a detailed page on [http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Software_Modelling_for_Synthetic_Biology modelling and iGEM. ] | Have a look at the Cambridge team wiki. They have a detailed page on [http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Software_Modelling_for_Synthetic_Biology modelling and iGEM. ] | ||
| - | *For everybody | + | *'''For everybody''' |
Have a look at the [https://2007.igem.org/Edinburgh Edinburgh team] wiki. They also present a Cre/Lox box in one of their constructions ! | Have a look at the [https://2007.igem.org/Edinburgh Edinburgh team] wiki. They also present a Cre/Lox box in one of their constructions ! | ||
| - | *Team Notebook and Protocols | + | *'''Team Notebook and Protocols''' |
I suggest that the Team Notebook section summarizes the work progress, the meaning of the different steps, and intermediate results. All protocols should be in the protocols section with links. | I suggest that the Team Notebook section summarizes the work progress, the meaning of the different steps, and intermediate results. All protocols should be in the protocols section with links. | ||
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''Answer'': click here to read [[Paris/Notebook#Monday, July 2|July 2nd]] entry. | ''Answer'': click here to read [[Paris/Notebook#Monday, July 2|July 2nd]] entry. | ||
| - | * | + | *''' Concerning the wiki''' |
The notebook is now accessible via [[Paris/Notebook_Calendar|a calendar]]. I hope that you will prefer the notebook like that. I moved some pages from their places to Paris/, and it is a good idea to take care that every page we create begins by Paris/... | The notebook is now accessible via [[Paris/Notebook_Calendar|a calendar]]. I hope that you will prefer the notebook like that. I moved some pages from their places to Paris/, and it is a good idea to take care that every page we create begins by Paris/... | ||
[[User:Nicolas C.|Nicolas C.]] | [[User:Nicolas C.|Nicolas C.]] | ||
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''[[User:Bottani| Samuel B.]]: The calender is a nice feature, however isn't it possible to have also everything in a single page. It is useful to have an overall view of the notebook. There is a risk with wikis to split informations on too many pages. This is clearly the case by presenting all in a calender...'' | ''[[User:Bottani| Samuel B.]]: The calender is a nice feature, however isn't it possible to have also everything in a single page. It is useful to have an overall view of the notebook. There is a risk with wikis to split informations on too many pages. This is clearly the case by presenting all in a calender...'' | ||
| - | *Have a look at the [https://2007.igem.org/Boston_University Boston team] wiki main page | + | I discussed with Eimad and David (P) and the idea is to make two notebooks : |
| + | * one accessible via the calendar, where we put all that we do during the day (still separated from protocols), for the people in the lab | ||
| + | * another one which will summarize goals and progresses step by step. We will begin this one soon. [[User:Nicolas C.|Nicolas C.]] | ||
| + | |||
| + | *'''Have a look at the [https://2007.igem.org/Boston_University Boston team] wiki main page''' | ||
I find it very clear. Maybe a good inspiration for a next version of ours... [[User:Bottani| Samuel B.]] | I find it very clear. Maybe a good inspiration for a next version of ours... [[User:Bottani| Samuel B.]] | ||
| + | |||
| + | *''' Concerning kinetic measurements ''' | ||
| + | I made a template in order to present growth experiments clearly when we use the 96 plate array. | ||
| + | Click here to see an [[Paris/July_3|example]]. | ||
Revision as of 20:54, 10 July 2007
Discussion Page
This page is for questions, answers and discussions on the project, a kind of open forum.
- Test of DapA deletion transduction, Thursday, July 5
How did you test for the success of this transduction? Simply with a normal culture without supplemented lysine in the medium, and seeing that the bacteria grows neverthless? Or is it that in the original DapA- strain tha DapA gene was replaced by an antibiotic resistance gene, that should then be present in the transfected bacteria?
- For the modellers
Have a look at the Cambridge team wiki. They have a detailed page on modelling and iGEM.
- For everybody
Have a look at the Edinburgh team wiki. They also present a Cre/Lox box in one of their constructions !
- Team Notebook and Protocols
I suggest that the Team Notebook section summarizes the work progress, the meaning of the different steps, and intermediate results. All protocols should be in the protocols section with links.
Question: how to link directly to a subsection of a page in a wiki?
Answer: click here to read July 2nd entry.
- Concerning the wiki
The notebook is now accessible via a calendar. I hope that you will prefer the notebook like that. I moved some pages from their places to Paris/, and it is a good idea to take care that every page we create begins by Paris/... Nicolas C.
Samuel B.: The calender is a nice feature, however isn't it possible to have also everything in a single page. It is useful to have an overall view of the notebook. There is a risk with wikis to split informations on too many pages. This is clearly the case by presenting all in a calender...
I discussed with Eimad and David (P) and the idea is to make two notebooks :
- one accessible via the calendar, where we put all that we do during the day (still separated from protocols), for the people in the lab
- another one which will summarize goals and progresses step by step. We will begin this one soon. Nicolas C.
- Have a look at the Boston team wiki main page
I find it very clear. Maybe a good inspiration for a next version of ours... Samuel B.
- Concerning kinetic measurements
I made a template in order to present growth experiments clearly when we use the 96 plate array. Click here to see an example.
