Paris/September 12

From 2007.igem.org

(Difference between revisions)
(DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR)
Line 11: Line 11:
- Primer2: 34: 2.5µL (final 0.5µM)<br>
- Primer2: 34: 2.5µL (final 0.5µM)<br>
-
[[Image:WannerDapA09122007.jpg|right|100px]]
+
[[Image:WannerDapA09122007.jpg|right|300px]]
* PCR Program:<br>
* PCR Program:<br>

Revision as of 14:34, 14 September 2007

DapA Wanner deletion: pKD3/pKD4-DapA homologies PCR

  • Material:

- pKD3/pKD4: 1 µL (30ng/60ng)
- Tampon: 10µL
- dNTP: 1µL (final 200µmol each)
- Taq: 0.5µL
- Water: 32.5µL
- Primer1: 33: 2.5µL (final 0.5µM)
- Primer2: 34: 2.5µL (final 0.5µM)

WannerDapA09122007.jpg
  • PCR Program:

35 cycles:
- 95°C: 5min
- 95°C: 30s (X35)
- 55°C: 30s (X35)
- 72°C: 1min30 (X35)
- 72°C: 10 min
- 4°C: inf

  • Quantity: ng/µL
  • Expected size: bp
  • After purification:

2µL of purification product (2µL of ladder)