PennState/Parts
From 2007.igem.org
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<a href="PART LINK HERE"><img border="0" src="http://openwetware.org/images/3/3e/Psuigem07picGene.jpg" width="121" height="121"></a></td> | <a href="PART LINK HERE"><img border="0" src="http://openwetware.org/images/3/3e/Psuigem07picGene.jpg" width="121" height="121"></a></td> | ||
- | <td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741023">CRP*</a></b><p><i>CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.</i><a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=CPLX0-226">[ | + | <td class="tddesc"><b><a href="http://partsregistry.org/Part:BBa_I741023">CRP*</a></b><p><i>CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.</i><a href="http://biocyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=CPLX0-226">[Hanamura92]</a></td> |
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+ | <h2>References</h2> | ||
+ | Hanamura92: Hanamura A, Aiba H (1992). "A new aspect of transcriptional control of the Escherichia coli crp gene: positive autoregulation." Mol Microbiol 1992;6(17);2489-97. PMID: 1328816 | ||
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Revision as of 20:37, 26 October 2007
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New Parts (submitted) |
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xylR XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons. |
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Promoter xylF The xylose F promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site. |
New Parts (underconstructed/unverified) |
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natural RBS xylR TT 'xylR total' XylR is a protein that belongs to the AraC/XylS family. This regulator participates in controlling several genes involved in xylose utilization. XylR activates the xylAB and xylFGHR operons. |
CRP* CRP* is a modified CRP which is cAMP independent. CRP is a dual transcriptional regulator that induces a severe bend in the DNA and is capable of regulating the expression of more than 200 genes. It is the major global regulator of catabolite-sensitive operons and it controls its own synthesis.[Hanamura92] |
Promoter xylA The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included as well as a CRP activator binding site. |
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Promoter xylA without CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working. |
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Promoter xylF without CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included, but the promoter region was cloned without the CRP binding site. Note: the removal of CRP may keep the promoter from working. |
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Two way promoter xylA-F with CRP site The xylose A promoter is activated by xylR-xylose, and may be negatively regulated by xylR. Two xylR halfsites are included and a CRP binding site. |