PennState/Project/Diauxie/PromoterTest

From 2007.igem.org

(Difference between revisions)
Line 12: Line 12:
After each of the four promoter constructs are tested individually, the two which include the CRP-cAMP are changed so that a gene expressing the mutant CRP* is included.
After each of the four promoter constructs are tested individually, the two which include the CRP-cAMP are changed so that a gene expressing the mutant CRP* is included.
-
<html><img src="http://openwetware.org/images/9/99/Pctestcrp.jpg" id="logo" name="logo" height="150" width="450" alt="" /></html>This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region followed by the CRP* gene.
+
<html><img src="http://openwetware.org/images/9/99/Pctestcrp.jpg" id="logo" name="logo" height="150" width="420" alt="" /></html>This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region followed by the CRP* gene.

Revision as of 21:39, 26 October 2007

Promoter Test Constructs

To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.


This construct uses the xylF and xylA facing promoters that includes the CRP-cAMP binding region.


This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region.


After each of the four promoter constructs are tested individually, the two which include the CRP-cAMP are changed so that a gene expressing the mutant CRP* is included.

This construct uses the xylF and xylA facing promoters that do not include the CRP-cAMP binding region followed by the CRP* gene.