PennState/Project/Diauxie/PromoterTest

From 2007.igem.org

< PennState | Project | Diauxie
Revision as of 21:26, 26 October 2007 by GTobin (Talk | contribs)

Promoter Test Constructs

To test each of the four xyl promoters, each is placed in a construct followed by a ribosome binding site, the reporter green florescent protein, and a terminator. The strength of each is measured by gfp emission.


This construct uses the xylF and xylA facing promoters that includes the CRP binding region.


This construct uses the xylA facing promoter that do not include the CRP binding region.


After each of the four promoter constructs are tested individually, the two