PennState/Project/Dosimeter/InitialTest
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| + | |[[PennState|Home]] | ||
| + | |[[PennState/Project/Diauxie|Diauxie Project]] | ||
| + | |[[PennState/Project/Dosimeter|Dosimeter Project]] | ||
| + | |[[PennState/People|People]] | ||
| + | |[[PennState/Lab|Lab]] | ||
| + | |[[PennState/Parts|Parts]] | ||
| + | |[[PennState/Acknowledgements|Acknowledgements]] | ||
| + | |[[PennState/Links|Links]] | ||
| + | |} | ||
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'''Dosimeter Test Construct''' | '''Dosimeter Test Construct''' | ||
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<html><img src="http://openwetware.org/images/5/54/Dosimeter_Test_Construct.jpg" id="logo" name="logo" height="128" width="800" alt="" /><br></html> | <html><img src="http://openwetware.org/images/5/54/Dosimeter_Test_Construct.jpg" id="logo" name="logo" height="128" width="800" alt="" /><br></html> | ||
This construct uses the lambda operator to express GFP when induced by radiation. | This construct uses the lambda operator to express GFP when induced by radiation. | ||
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| + | {| border="0" cellspacing="8px" cellpadding="15" width="100%" | ||
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| + | |[[PennState/Project/Dosimeter|Dosimeter Introduction]] | ||
| + | |[[PennState/Project/Dosimeter/InitialTest|Test Constructs]] | ||
| + | |[[PennState/Project/Dosimeter/Dosimetry|Dosimetry]] | ||
| + | |[[PennState/Project/Dosimeter/FinalConstructs|Final Constructs]] | ||
| + | |} | ||
Revision as of 22:38, 26 October 2007
| Home | Diauxie Project | Dosimeter Project | People | Lab | Parts | Acknowledgements | Links |
Dosimeter Test Construct
To test the dosimeter, the assembled lambda region is placed in a plasmid preceding a ribosome binding site, the reporter green florescent protein, and a terminator. The promoter's sensitivity to radiation can be readily tested with flow cell cytometry and fluoresence plate reading
This construct uses the lambda operator to express GFP when induced by radiation.
| Dosimeter Introduction | Test Constructs | Dosimetry | Final Constructs |
